Evaluation of a Rapid Whole Blood ELISA for Quantification of Troponin I in Patients with Acute Chest Pain

Troponin I (cTnI) provides important prognostic information in patients with chest pain. We wished to evaluate a rapid, whole-blood analyzer for quantitative point-of-care testing. A quantitative point-of-care test system (Stratus CS((R)); Dade-Behring) for cTnI with an incorporated centrifuge was e...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1999-10, Vol.45 (10), p.1789-1796
Main Authors: Heeschen, Christopher, Goldmann, Britta U, Langenbrink, Lukas, Matschuck, Guido, Hamm, Christian W
Format: Article
Language:English
Subjects:
Acute Disease
Angina, Unstable - diagnosis
Biological and medical sciences
Biomarkers - blood
Cardiovascular system
Chest Pain - diagnosis
Enzyme-Linked Immunosorbent Assay
Female
Fluoroimmunoassay
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Middle Aged
Myocardial Infarction - diagnosis
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Point-of-Care Systems
ROC Curve
Troponin I - blood
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Summary:Troponin I (cTnI) provides important prognostic information in patients with chest pain. We wished to evaluate a rapid, whole-blood analyzer for quantitative point-of-care testing. A quantitative point-of-care test system (Stratus CS((R)); Dade-Behring) for cTnI with an incorporated centrifuge was evaluated in 412 patients with chest pain less than 12 h. Results were available within 15 min. CVs were 4.5% at 0.1 microgram/L, 4.2% at 0.25 microgram/L, and 6.5% at 0.82 microgram/L. The detection limit was 0. 01 microgram/L. The 97.5% percentile in a healthy population was 0.08 microgram/L. Based on ROC curve analysis, a threshold of 0.15 microgram/L was calculated for the detection of acute myocardial infarction (AMI). With it, sensitivity for the detection of patients with AMI (n = 62) was 63% at arrival and 98% after 4 h (Stratus II((R)), 48% and 85%, respectively; P /=0.08 microgram/L (Stratus II, 28%; P
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/45.10.1789