Discrimination between pentose oligosaccharides containing D-xylopyranose or L-arabinofuranose as non-reducing terminal residue using fast atom bombardment mass spectrometry

• Published in: Journal of mass spectrometry 2001-04, Vol.36 (4), p.379-383
• Main Authors: Kováčik, Vladimír, Pätoprstý, Vladimír, Hirsch, Ján
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Arabinose - analogs & derivatives; Arabinose - analysis; Biological and medical sciences; Carbohydrates; Chemistry; Collision-induced dissociation tandem mass spectrometry; D-xylopyranosyl and/or L-arabinofuranosyl non-reducing terminal residues; Exact sciences and technology; fast atom bombardment; Fundamental and applied biological sciences. Psychology; Holosides; Mass spectrometry; oligosaccharides; Oligosaccharides - analysis; Organic chemistry; Other biological molecules; Reactivity and mechanisms; ring size estimation; Spectrometry, Mass, Fast Atom Bombardment - methods; Xylose - analogs & derivatives; Xylose - analysis
• ISSN: 1076-5174; 1096-9888
• DOI: 10.1002/jms.138

Collisional‐induced dissociation (CID) mass spectra of the [M + H]+ and [M − H]− ions obtained under fast atom bombardment conditions of a number of methyl glycoside di‐, tri‐ and tetrasaccharides, containing D‐xylopyranosyl and/or L‐arabinofuranosyl residues at the non‐reducing terminus, do not provide information about their ring size. This information could only be obtained from a careful comparison of the intensity ratio of the [M + Na − 90]+ and [M + Na − 104]+ ions (0,2Xt/1,5Xt) in the high‐energy CID spectra of the sodium‐cationized di‐, tri‐ and probably also tetrasaccharide compounds. Copyright © 2001 John Wiley & Sons, Ltd.