Selective recognition of a C-G base-pair in the parallel DNA triple-helical binding motif

Selective recognition of a C-G base-pair within the parallel DNA triple-helical binding motif was achieved by a third strand containing the base 5-methyl pyrimidin-2-one. The third strand affinities (K D) for a representative 15-mer duplex sequence containing all four Watson-Crick base pairs (X-Y) i...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 1999-09, Vol.9 (18), p.2657-2660
Main Authors: Prévot-Halter, Isabelle, Leumann, Christian J.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Pairing
Base Sequence
Binding Sites
Biological and medical sciences
DNA - chemistry
DNA - metabolism
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
Nucleic acids
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Summary:Selective recognition of a C-G base-pair within the parallel DNA triple-helical binding motif was achieved by a third strand containing the base 5-methyl pyrimidin-2-one. The third strand affinities (K D) for a representative 15-mer duplex sequence containing all four Watson-Crick base pairs (X-Y) in the center are C-G (26 nM) ⪢ A-T (270 nM) ∼ T-A (350 nM) > G-C (ca. 700 nM). C-G base-pair recognition was achieved by third strands containing the base 5-methyl pyrimidin-2-one.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(99)00451-5