Characterization of a Novel cDNA Sequence Encoding Invertebrate Tachykinin-Related Peptides Isolated from the Echiuroid Worm, Urechis unicinctus

Tachykinin is one of the most well-known bioactive peptides found in vertebrates, and tachykinin-related peptides have also been isolated from various invertebrate species. Urechistachykinin I (Leu-Arg-Gln-Ser-Gln-Phe-Val-Gly-Ser-Arg-NH2) and II (Ala-Ala-Gly-Met-Gly-Phe-Phe-Gly-Ala-Arg-NH2) were pur...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-10, Vol.263 (3), p.848-852
Main Authors: Kawada, Tsuyoshi, Satake, Honoo, Minakata, Hiroyuki, Muneoka, Yojiro, Nomoto, Kyosuke
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Annelida - genetics
Base Sequence
Cloning, Molecular
DNA Primers
DNA, Complementary
Molecular Sequence Data
neuropeptide
Neuropeptides - chemistry
Neuropeptides - genetics
Open Reading Frames
precursor
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Sequence Alignment
Sequence Homology, Amino Acid
tachykinin
Urechis unicinctus
Uru-TK
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Summary:Tachykinin is one of the most well-known bioactive peptides found in vertebrates, and tachykinin-related peptides have also been isolated from various invertebrate species. Urechistachykinin I (Leu-Arg-Gln-Ser-Gln-Phe-Val-Gly-Ser-Arg-NH2) and II (Ala-Ala-Gly-Met-Gly-Phe-Phe-Gly-Ala-Arg-NH2) were purified from the ventral nerve cords of echiuroid worm, Urechis unicinctus. In the present study, we described the characterization of a novel cDNA encoding the urechistachykinin precursor. Amino acid sequence analysis of the deduced polypeptide revealed that the urechistachykinin precursor included seven structurally related peptides, unlike mammalian tachykinin precursors which encode only one or two tachykinin peptides. This is the first identification of an invertebrate tachykinin-related peptide cDNA.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.1465