Effects of amino acid substitutions at conserved and acidic residues within region 1.1 of Escherichia coli sigma(70)

Amino acid substitutions in Escherichia coli sigma(70) were generated and characterized in an analysis of the role of region 1.1 in transcription initiation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivit...

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Bibliographic Details
Published in:Journal of bacteriology 2000-01, Vol.182 (1), p.221
Main Authors: Bowers, C W, McCracken, A, Dombroski, A J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution - genetics
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Conserved Sequence
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Escherichia coli - chemistry
Escherichia coli - genetics
Mutagenesis, Site-Directed
RNA, Bacterial - biosynthesis
Sigma Factor - genetics
Sigma Factor - metabolism
Structure-Activity Relationship
Transcription, Genetic
Trypsin - metabolism
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Description
Summary:Amino acid substitutions in Escherichia coli sigma(70) were generated and characterized in an analysis of the role of region 1.1 in transcription initiation. Several acidic and conserved residues are tolerant of substitution. However, replacement of aspartic acid 61 with alanine results in inactivity caused by structural and functional thermolability.
ISSN:0021-9193
DOI:10.1128/JB.182.1.221-224.2000