Purification and Protein Composition of PM2, the First Lipid-Containing Bacterial Virus To Be Isolated

The marine, icosahedral bacteriophage PM2 was isolated in the late 1960s. It was the first phage for which lipids were firmly demonstrated to be part of the virion structure and it has been classified as the type organism of the Corticoviridae family. The host, Pseudoalteromonas espejiana BAL-31, be...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1999-09, Vol.262 (2), p.364-374
Main Authors: Kivelä, Hanna M., Männistö, Riina H., Kalkkinen, Nisse, Bamford, Dennis H.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteria - classification
Bacteria - virology
Calcium Chloride - pharmacology
Centrifugation, Zonal
Cesium
Chlorides
Corticoviridae - chemistry
Corticoviridae - drug effects
Corticoviridae - growth & development
Corticoviridae - isolation & purification
Genome, Viral
Glycerol
Immune Sera - pharmacology
infectivity
Lipids - analysis
Molecular Sequence Data
Molecular Weight
Phage pm2
PM2 bacteriophage
Pseudoalteromonas espejiana
purification
RNA, Ribosomal, 16S - analysis
RNA, Viral - analysis
Sequence Analysis
Sodium Chloride - pharmacology
structural proteins
Sucrose
Time Factors
Triiodobenzoic Acids
Viral Structural Proteins - chemistry
Viral Structural Proteins - genetics
Viral Structural Proteins - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The marine, icosahedral bacteriophage PM2 was isolated in the late 1960s. It was the first phage for which lipids were firmly demonstrated to be part of the virion structure and it has been classified as the type organism of the Corticoviridae family. The host, Pseudoalteromonas espejiana BAL-31, belongs to a common group of marine bacteria. We developed a purification method producing virions with specific infectivity approximately as high as that of the lipid-containing phages PRD1 and φ6. The sensitivity of the virus to normally used purification media such as those containing sucrose is demonstrated. We also present an alternative host, a pseudoalteromonad, that allows enhanced purification of the virus under reduced salt conditions. We show, using N-terminal amino acid sequencing and comparison with the genomic sequence, that there are at least eight structural proteins in the infectious virus.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1999.9838