Calcium imaging with chemiluminescence

This review updates the imaging of free cytosolic calcium with the chemiluminescent aequorins. Basic principles of chemiluminescence are discussed and the biochemistry of aequorins is briefly described. The review provides practical tips on handling and microinjecting aequorins and describes availab...

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Bibliographic Details
Published in:Microscopy research and technique 1999-09, Vol.46 (6), p.390-397
Main Authors: Créton, Robbert, Kreiling, Jill A., Jaffe, Lionel F.
Format: Article
Language:English
Subjects:
aequorin
Aequorin - metabolism
Animals
Ca2
Calcium - analysis
Calibration
Cell Size
coelenterazine
detector
fluorescence
Humans
luminescence
Luminescent Measurements
Microinjections
photon
photoprotein
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Summary:This review updates the imaging of free cytosolic calcium with the chemiluminescent aequorins. Basic principles of chemiluminescence are discussed and the biochemistry of aequorins is briefly described. The review provides practical tips on handling and microinjecting aequorins and describes available ultra low light imaging systems. It is argued that aequorin‐based calcium imaging is the method of choice for exploratory studies, since it is extremely sensitive, can detect a broad range of calcium concentrations, and allows for continuous recording during long periods of time. However, fluorescent methods are needed to attain high spatial resolution. Microsc. Res. Tech. 46:390–397, 1999. © 1999 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/(SICI)1097-0029(19990915)46:6<390::AID-JEMT7>3.0.CO;2-S