Clonal Relationships and Extended Virulence Genotypes among Escherichia coli Isolates from Women with a First or Recurrent Episode of Cystitis

To identify bacterial predictors of recurrence and/or persistence in acute cystitis, extended virulence genotypes were compared with clonal background and epidemiologic status among 74 Escherichia coli urine isolates from women with first or recurrent episodes of urinary tract infection (UTI). Seque...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 2001-05, Vol.183 (10), p.1508-1517
Main Authors: Johnson, James R., O’Bryan, Timothy T., Delavari, Parissa, Kuskowski, Michael, Stapleton, Ann, Carlino, Ulrike, Russo, Thomas A.
Format: Article
Language:English
Subjects:
Acute Disease
Alleles
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacteriology
Biological and medical sciences
Clone Cells
Cystitis
Cystitis - epidemiology
Cystitis - microbiology
Escherichia coli
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Female
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genotype
Genotypes
Humans
Major Articles
Medical sciences
Microbiology
papA gene
papG gene
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pharmacology. Drug treatments
Phenotypic traits
Phylogenetics
Phylogeny
Recurrence
Urinary tract infections
Urine
Urine - microbiology
Virulence
Virulence factors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To identify bacterial predictors of recurrence and/or persistence in acute cystitis, extended virulence genotypes were compared with clonal background and epidemiologic status among 74 Escherichia coli urine isolates from women with first or recurrent episodes of urinary tract infection (UTI). Sequential isolates from patients with recurrent UTI were classified, using macrorestriction analysis, as having caused an isolated recurrence versus a single or multiple same-strain recurrences. papA, papG allele II, iha and iutA predicted multiple same-strain recurrences, whereas nfaE and the absence of sfaS or fyuA predicted isolated recurrences. Phylogenetic group B2 accounted for 70% of isolates and for most of the putative virulence factors (VFs) studied. The meningitis-associated O18:K1:H7 clonal group comprised 18% of isolates, exhibited multiple VFs, and caused “once-only” recurrences less commonly than did other strains. These findings identify specific VFs and clonal groups against which preventive interventions might be beneficial and illustrate the importance of delineating pathogenetically relevant subgroups within the “recurrent cystitis” population
ISSN:0022-1899
1537-6613
DOI:10.1086/320198