Wide‐field optically sectioning fluorescence microscopy with laser illumination

We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one‐dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an im...

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Published in:Journal of microscopy (Oxford) 2000-01, Vol.197 (1), p.1-4
Main Authors: Neil, M A, Squire, A, Juskaitis, R, Bastiaens, P I, Wilson, T
Format: Article
Language:English
Subjects:
Animals
COS Cells - cytology
COS Cells - metabolism
Fluorescence microscopy
Image Enhancement
Isoenzymes - metabolism
Lasers
Microscopy, Confocal - methods
Microscopy, Fluorescence - methods
optical sectioning
Protein Kinase C - metabolism
Protein Kinase C-alpha
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description We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one‐dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.
doi_str_mv 10.1046/j.1365-2818.2000.00656.x
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subjects Animals
COS Cells - cytology
COS Cells - metabolism
Fluorescence microscopy
Image Enhancement
Isoenzymes - metabolism
Lasers
Microscopy, Confocal - methods
Microscopy, Fluorescence - methods
optical sectioning
Protein Kinase C - metabolism
Protein Kinase C-alpha
title Wide‐field optically sectioning fluorescence microscopy with laser illumination
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