Persistent infection of a lymphoma cell line by herpes simplex virus

The peripheral blood cells from a patient with a B‐cell lymphoma were established in long‐term tissue culture. Two years after establishment of the cells in culture they were infected with herpes simplex virus type 2 and the productivity and duration of viral persistence investigated. One week after...

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Bibliographic Details
Published in:American journal of hematology 1999-10, Vol.62 (2), p.93-98
Main Authors: Kao, Yuan S., Sundin, Daniel R., Gebhardt, Bryan M.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
cell culture
Cell Culture Techniques - methods
Cell Division
Cell Line
Chromosome Aberrations
Chromosome Banding
Hematologic and hematopoietic diseases
herpes simplex virus type 2
Herpesvirus 2, Human - isolation & purification
Herpesvirus 2, Human - physiology
Humans
karyotype
Karyotyping
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
lymphoma
Lymphoma, B-Cell - blood
Lymphoma, B-Cell - genetics
Lymphoma, B-Cell - pathology
Lymphoma, B-Cell - virology
Male
Medical sciences
persistent infection
Polymerase Chain Reaction
Tumor Cells, Cultured
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Summary:The peripheral blood cells from a patient with a B‐cell lymphoma were established in long‐term tissue culture. Two years after establishment of the cells in culture they were infected with herpes simplex virus type 2 and the productivity and duration of viral persistence investigated. One week after infection the lymphoblastoid cells were productively infected and have remained so for a period of over 3 years. Expression of a viral glycoprotein antigen was evaluated by using a fluorescein‐labeled monoclonal anti‐herpes simplex virus type 2 antibody and revealed a spectrum of staining reactions grading from a lightly stippled to very intense pattern. Polymerase chain reaction analysis of the infected cells revealed the presence of the herpes simplex virus type 2 DNA polymerase gene in the infected cells that was absent from the uninfected lymphoblastoid cells. These results taken together with the long‐term growth characteristics of both the infected and uninfected lymphoblastoid cells suggest that this cell line may be a good model system for studying viral infection, viral replication, viral latency, and clinical application for the isolation of human herpes virus. Am. J. Hematol. 62:93–98, 1999. © 1999 Wiley‐Liss, Inc.
ISSN:0361-8609
1096-8652
DOI:10.1002/(SICI)1096-8652(199910)62:2<93::AID-AJH5>3.0.CO;2-7