Photoelectric response of the N intermediate of bacteriorhodopsin and its mutant T46V

Double flash experiments were performed in order to gain information about the characteristics of the N intermediates of the photocycle of bacteriorhodopsin. The N intermediates of wild-type bacteriorhodopsin and mutant T46V were excited at different delay times after the first laser flash which ind...

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Bibliographic Details
Published in:FEBS letters 1999-10, Vol.459 (1), p.5-8
Main Authors: Tóth-Boconádi, R., Szabó-Nagy, A., Taneva, S.G., Keszthelyi, L.
Format: Article
Language:English
Subjects:
Bacteriorhodopsin mutant
Bacteriorhodopsins - chemistry
Bacteriorhodopsins - genetics
Bacteriorhodopsins - physiology
Double flash excitation
Electrophysiology
Halobacterium salinarum - genetics
Halobacterium salinarum - physiology
Mutation
Oriented purple membrane
Photochemistry
Proton pumping
Proton Pumps - metabolism
Purple Membrane - physiology
Retinal protein
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Summary:Double flash experiments were performed in order to gain information about the characteristics of the N intermediates of the photocycle of bacteriorhodopsin. The N intermediates of wild-type bacteriorhodopsin and mutant T46V were excited at different delay times after the first laser flash which induced the photocycle and the electric responses were registered. These electric signals revealed that charge motions occurred in both cases, though charge translocation, i.e. H + pumping, could not be observed. The delay time dependence of the electric signals is characterized by two distinct processes corresponding to two substates of the N intermediates.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)01192-8