IL-12 as well as IL-2 upregulates CCR5 expression on T cell receptor-triggered human CD4+ and CD8+ T cells

The expression of chemokine receptors on leukocytes is related to their activation state. However, the exact mechanism underlying the induction of each chemokine receptor is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell trafficking and HIV infection, is...

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Bibliographic Details
Published in:Journal of clinical immunology 2001-03, Vol.21 (2), p.116-125
Main Authors: YANG, Yi-Fu, TOMURA, Michio, IWASAKI, Masayuki, MUKAI, Takao, PING GAO, ONO, Shiro, ZOU, Jian-Ping, SHEARER, Gene M, FUJIWARA, Hiromi, HAMAOKA, Toshiyuki
Format: Article
Language:English
Subjects:
Biological and medical sciences
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cells, Cultured
Fundamental and applied biological sciences. Psychology
Humans
Inflammation
Interleukin-12 - immunology
Interleukin-12 - pharmacology
Interleukin-2 - immunology
Interleukin-2 - pharmacology
Molecular and cellular biology
Receptors, Antigen, T-Cell - immunology
Receptors, CCR5 - immunology
Signal Transduction - immunology
Up-Regulation - drug effects
Up-Regulation - immunology
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Summary:The expression of chemokine receptors on leukocytes is related to their activation state. However, the exact mechanism underlying the induction of each chemokine receptor is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell trafficking and HIV infection, is induced in human T cells. CCR5 was marginally detected on a freshly prepared human peripheral blood mononuclear cell (PBMC) population. Long-term (8-day) stimulation of PBMC with IL-2 resulted in high levels of CCR5 expression on T cells. IL-12 failed to induce CCR5 on T cells in such a directly stimulated PBMC population. Stimulation of PBMC T cells with anti-CD3 plus anti-CD28 induced detectable albeit very low levels of CCR5 along with the induction of IL-12 receptor. However, these TCR-triggered T cells expressed much higher levels of CCR5 when stimulated with IL-12. Although IL-2 also induced CCR5 expression, CCR5 expression was more potent in IL-12 than IL-2 stimulation. These results indicate that, in addition to IL-2, IL-12 plays an important role in the induction of CCR5 expression on T cells, particularly TCR-triggered T cells.
ISSN:0271-9142
1573-2592
DOI:10.1023/A:1011059906777