Co-adsorbed fibrinogen and von Willebrand factor augment platelet procoagulant activity and spreading

Previously we observed that platelets adherent to surfaces preadsorbed with blood plasma exhibited 1.3 to 2.4 times greater procoagulant activity than platelets on surfaces adsorbed with fibrinogen (Fg) only. These observations suggested that the adhesion proteins adsorbed from plasma may activate p...

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Bibliographic Details
Published in:Journal of biomaterials science. Polymer ed. 2001-01, Vol.12 (1), p.1-20
Main Authors: Grunkemeier, J. M., Tsai, W. B., Horbett, T. A.
Format: Article
Language:English
Subjects:
Blood Platelets - metabolism
Blood Platelets - ultrastructure
Cell Adhesion - drug effects
Cell Movement - drug effects
Coagulants - chemistry
Dose-Response Relationship, Drug
FIBRINOGEN
Fibrinogen - chemistry
Humans
L-Lactate Dehydrogenase - blood
Microscopy, Electron, Scanning
Plasma - chemistry
PLATELET ADHESION
Platelet Aggregation - drug effects
Polystyrenes - chemistry
PROCOAGULANT ACTIVITY
THROMBIN
Thromboplastin - metabolism
von Willebrand Factor - chemistry
VWF
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Summary:Previously we observed that platelets adherent to surfaces preadsorbed with blood plasma exhibited 1.3 to 2.4 times greater procoagulant activity than platelets on surfaces adsorbed with fibrinogen (Fg) only. These observations suggested that the adhesion proteins adsorbed from plasma may activate platelets in a cooperative, or synergistic manner. In the present study, polystyrene surfaces adsorbed with both Fg and vWF induced up to three times greater procoagulant activity than surfaces adsorbed with Fg or vWF only. The amounts of Fg and vWF adsorbed from binary mixtures that resulted in increased procoagulant activity were found to be similar to the amounts that adsorbed to PS from 100% plasma. The effect of adsorbed adhesion proteins on platelet spreading was also investigated. The proportion of fully spread platelets increased, depending on the adhesion protein preadsorbed to the surface, in the following order: vWF < Fg < Fn < (vWF + Fg) < Vn < plasma.
ISSN:0920-5063
1568-5624
DOI:10.1163/156856201744416