The chondrogenic potential of periosteum decreases with age

Periosteum contains undifferentiated mesenchymal stem cells that possess the potential for chondrogenesis during cartilage repair and in fracture healing. With aging, the chondrogenic potential of periosteum declines significantly. An organ-culture model was used to investigate the relationship betw...

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Bibliographic Details
Published in:Journal of orthopaedic research 2001, Vol.19 (1), p.95-103
Main Authors: O'Driscoll, Shawn W.M, Saris, Daniel B.F, Ito, Yoichi, Fitzimmons, James S
Format: Article
Language:English
Subjects:
Age Factors
Animals
Bone Development
Cartilage - growth & development
Cell Division
Glycosaminoglycans - biosynthesis
Periosteum - growth & development
Rabbits
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Summary:Periosteum contains undifferentiated mesenchymal stem cells that possess the potential for chondrogenesis during cartilage repair and in fracture healing. With aging, the chondrogenic potential of periosteum declines significantly. An organ-culture model was used to investigate the relationship between the chondrogenic potential of periosteum and aging. A total of 736 periosteal explants from the proximal medial tibiae of 82 rabbits, aged 2 weeks to 2 years, were cultured in agarose suspension conditions conductive for chondrogenesis, and analyzed using histomorphometry, collagen typing, wet weight measurement, 3H-thymidine and 35S-sulfate uptake, autoradiography, and PCNA immunostaining. The rabbits were skeletally mature by 6 months and stopped increasing in weight by 12 months. Chondrogenesis declined significantly with age ( P
ISSN:0736-0266
1554-527X
DOI:10.1016/S0736-0266(00)00014-0