Monolithic Capillary Electrophoresis Device with Integrated Fluorescence Detector

A monolithic capillary electrophoresis system with integrated on-chip fluorescence detector has been microfabricated on a silicon substrate. Photodiodes in the silicon substrate measure fluorescence emitted from eluting molecules. The device incorporates an on-chip thin-film interference filter that...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-04, Vol.73 (7), p.1622-1626
Main Authors: Webster, J. R, Burns, M. A, Burke, D. T, Mastrangelo, C. H
Format: Article
Language:English
Subjects:
Analytical chemistry
Bacteriophage phi X 174 - genetics
Benzoxazoles
Blood vessels
Chemistry
Chromatographic methods and physical methods associated with chromatography
DNA
DNA, Viral - analysis
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Exact sciences and technology
Fluorescence
Fluorescent Dyes
Fluorometry - instrumentation
Fluorometry - methods
Measurement
Other chromatographic methods
Photodetectors
Photodiodes
Quinolinium Compounds
Silicon
Substrates
Thin film devices
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Summary:A monolithic capillary electrophoresis system with integrated on-chip fluorescence detector has been microfabricated on a silicon substrate. Photodiodes in the silicon substrate measure fluorescence emitted from eluting molecules. The device incorporates an on-chip thin-film interference filter that prevents excitation light from inhibiting the fluorescence detection. A transparent AZO conducting ground plane is also used to prevent the high electric fields used for the separation from interfering with the photodiode response. Separations of DNA restriction fragments have been performed in these devices with femtogram detection limits using SYBR Green I intercalating dye.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0004512