Significant Correlation between Interleukin 10 Expression and Vascularization through Angiopoietin/TIE2 Networks in Non-small Cell Lung Cancer

• Published in: Clinical cancer research 2001-05, Vol.7 (5), p.1287-1292
• Main Authors: HATANAKA, Hiroyuki, ABE, Yoshiyuki, KIJIMA, Hiroshi, YAMAZAKI, Hitoshi, INOUE, Hiroshi, UEYAMA, Yoshito, NAKAMURA, Masato, NARUKE, Masao, TOKUNAGA, Tetsuji, OSHIKA, Yoshiro, KAWAKAMI, Tsutomu, OSADA, Hideo, NAGATA, Junko, KAMOCHI, Jun-Ichiro, TSUCHIDA, Takashi
• Format: Article
• Language: English
• Subjects: Angiogenesis Inhibitors; Angiogenic Proteins; Angiopoietin-2; Biological and medical sciences; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; DNA-Binding Proteins; Endothelial Growth Factors - metabolism; Humans; Interleukin-10 - biosynthesis; Interleukin-10 - metabolism; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Lymphokines - metabolism; Medical sciences; Neoplasm Staging; Neovascularization, Pathologic - metabolism; Pneumology; Proteins - metabolism; Receptors, Interleukin - biosynthesis; Receptors, Interleukin-10; Saccharomyces cerevisiae Proteins; Statistics as Topic; Thrombospondins - metabolism; Transcription Factors; Tumors of the respiratory system and mediastinum; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
• ISSN: 1078-0432; 1557-3265

The expression of interleukin 10 (IL-10) is correlated with clinical prognosis in non-small cell lung cancer [NSCLC (H. Hatanaka et al. , Ann. Oncol., 11: 815–819, 2000)]. However, the effects of IL-10 expression on vascularization in NSCLC are not apparent. We examined the gene expression of IL-10/IL-10 receptor and various angiogenic/angioinhibitory factors in 95 NSCLC samples to determine the correlation between IL-10 production and vascularization. Vascular endothelial growth factor, angiopoietin [ Ang ( Ang-1 and Ang-2 )], thrombospondin, brain-specific angiogenesis inhibitor 1, vascular endothelial growth factor receptors ( KDR and flt-1 ), and Ang receptor ( TIE2 ) gene expression were evaluated by reverse transcription-PCR. The cellular localization of these factors and vascularity in the cancer stroma were examined immunohistochemically. Seventy-eight (82.1%) and 93 (97.9%) of these 95 NSCLCs were positive for IL-10 and IL-10 receptor, respectively. Ang-1 , Ang-2 , and TIE2 gene expression was seen in 76 (97.4%), 73 (93.6%), and 78 (100%) of 78 IL-10-positive NSCLCs, respectively, and was significantly correlated with IL-10 gene expression ( P < 0.0088,