Analysis of pigment accumulation heterogeneity in plant cell population by image-processing system

Plant‐cultured cells are often highly heterogeneous in secondary metabolite productivity. The industrial application for large‐scale metabolite production requires establishment of a stable high‐producing cell line. In this study, image analysis of the individual cell is investigated as a method for...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2000-02, Vol.67 (4), p.493-497
Main Authors: Miyanaga, Kazuhiko, Seki, Minoru, Furusaki, Shintaro
Format: Article
Language:English
Subjects:
anthocyanin
Anthocyanins - analysis
Anthocyanins - metabolism
Batch cell culture
Biological and medical sciences
Biotechnology
Cell Division
Estimation
Eukaryotic cell cultures
Fruits
Fundamental and applied biological sciences. Psychology
heterogeneous cell population
Image analysis
Image Processing, Computer-Assisted
image-processing system
Imaging systems
Methods. Procedures. Technologies
Microscopy
Miscellaneous
Pigments
Pigments, Biological - analysis
Pigments, Biological - metabolism
plant cell culture
Plant cells and fungal cells
Plants, Edible - cytology
Plants, Edible - metabolism
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Summary:Plant‐cultured cells are often highly heterogeneous in secondary metabolite productivity. The industrial application for large‐scale metabolite production requires establishment of a stable high‐producing cell line. In this study, image analysis of the individual cell is investigated as a method for evaluation of a heterogeneous cell population, and compared with the conventional method of estimation, which is based on average‐cell productivity. Among strawberry cells producing anthocyanins, cells with a wide‐range of pigment concentration were observed and maximum anthocyanin content was 10 times higher than the average value. In addition, a change of the frequency distribution was revealed in batch cultivation. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 67: 493–497, 2000.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(20000220)67:4<493::AID-BIT13>3.0.CO;2-U