The molecular characterization and in situ expression pattern of pea [Pisum sativum] SCARECROW gene

Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for grav...

Full description

Saved in:
Bibliographic Details
Published in:Plant and cell physiology 2001-04, Vol.42 (4), p.385-394
Main Authors: Sassa, N. (Japan Women's Univ., Tokyo), Matsushita, Y, Nakamura, T, Nyunoya, H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - genetics
Arabidopsis Proteins
Biological Evolution
CELL STRUCTURE
Cloning, Molecular
Conserved Sequence
DNA Primers
endodermis
GENE EXPRESSION
Gene Expression Regulation, Plant
Gene Library
Genetic Variation
gravitropism
Leucine Zippers
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
PISUM SATIVUM
Pisum sativum - classification
Pisum sativum - genetics
Plant Proteins - genetics
Plant Shoots - physiology
SCARECROW gene
SCR gene
Sequence Alignment
Sequence Homology, Amino Acid
TROPISMS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabidopsis thaliana. The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library; Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues. A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct frorn the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR. A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript. Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pce048