Drosophila wingless and Pair-Rule Transcripts Localize Apically by Dynein-Mediated Transport of RNA Particles

Asymmetric mRNA localization targets proteins to their cytoplasmic site of function. We have elucidated the mechanism of apical localization of wingless and pair-rule transcripts in the Drosophila blastoderm embryo by directly visualizing intermediates along the entire path of transcript movement. A...

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Bibliographic Details
Published in:Cell 2001-04, Vol.105 (2), p.209-219
Main Authors: Wilkie, Gavin S., Davis, Ilan
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - drug effects
Active Transport, Cell Nucleus - physiology
Animals
Antineoplastic Agents, Phytogenic - pharmacology
Cell Polarity
Demecolcine - pharmacology
DNA-Binding Proteins - metabolism
Drosophila
Drosophila melanogaster - physiology
Drosophila Proteins
Dyneins - genetics
Dyneins - metabolism
Embryo, Nonmammalian - cytology
Embryo, Nonmammalian - physiology
Fluorescent Dyes - metabolism
Fushi Tarazu Transcription Factors
Homeodomain Proteins - metabolism
In Situ Hybridization, Fluorescence
Microinjections
Microscopy, Fluorescence
Microtubules - metabolism
Nuclear Proteins
Proto-Oncogene Proteins - metabolism
RNA, Messenger - metabolism
Time Factors
Transcription Factors
wingless gene
Wnt1 Protein
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Summary:Asymmetric mRNA localization targets proteins to their cytoplasmic site of function. We have elucidated the mechanism of apical localization of wingless and pair-rule transcripts in the Drosophila blastoderm embryo by directly visualizing intermediates along the entire path of transcript movement. After release from their site of transcription, mRNAs diffuse within the nucleus and are exported to all parts of the cytoplasm, regardless of their cytoplasmic destinations. Endogenous and injected apical RNAs assemble selectively into cytoplasmic particles that are transported apically along microtubules. Cytoplasmic dynein is required for correct localization of endogenous transcripts and apical movement of injected RNA particles. We propose that dynein-dependent movement of RNA particles is a widely deployed mechanism for mRNA localization.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(01)00312-9