TESTICULAR SPERM ASPIRATION (TESA) AND ITS APPLICATION IN OOCYTE DONATION

• Published in: Archives of andrology 2001, Vol.46 (3), p.211-215
• Main Authors: LINDHEIM, S. R, CRUMM, K, FISCH, H, SAUER, M. V
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Birth control; Cell Separation; Female; Fertilization in Vitro; Gynecology. Andrology. Obstetrics; Humans; Infertility, Male; Male; Medical sciences; Middle Aged; Oocyte Donation; Oocyte Donation Pregnancy Sperm Tesa Testes; Outcome and Process Assessment (Health Care); Pregnancy; Spermatozoa; Sterility. Assisted procreation; Suction; Testis - cytology; Tissue Donors
• ISSN: 0955-3002; 0148-5016; 1362-3095; 1521-0375
• DOI: 10.1080/01485010151096522

This study was conducted to assess the outcomes of TESA in women undergoing oocyte donation. Descriptive reports from a university IVF practice were reviewed to evaluate the outcomes of women (n = 10) who underwent 12 cycles of ovum donation and required testicular sperm aspiration due to obstructive azoospermia (n = 6) and nonobstructive azoospermia (n = 4). Percutaneous needle aspiration of the testes was performed prior to oocyte retrieval to recover sperm, and intracytoplasmic sperm injection was subsequently performed on the donated oocytes. Fertilization was documented 16-18 h later and transcervical embryo transfer performed 72 h postretrieval. Successful retrieval of testicular spermatozoa utilizing the percutaneous technique was achieved in all 12 cycles. All cycles resulted in an ET with the achievement of 7 (58%) pregnancies, of which 5 (42%) are ongoing or delivered. The individual embryo implantation rate was 18.4% (9/49). Two cycles (16.6%) produced supernumerary embryos for cryopreservation. TESA represents a simple procurement technique associated with minimal morbidity. It may be offered even in cases of nondestructive azoospermia.