Structural and serological studies on Hafnia alvei O-specific polysaccharide of α-d-mannan type isolated from the lipopolysaccharide of strain PCM 1223

• Published in: FEMS immunology and medical microbiology 2001-04, Vol.30 (3), p.223-227
• Main Authors: Katzenellenbogen, Ewa, Kocharova, Nina A., Zatonsky, George V., Kübler-Kielb, Joanna, Gamian, Andrzej, Shashkov, Alexander S., Knirel, Yurij A., Romanowska, Elżbieta
• Format: Article
• Language: English
• Subjects: Chromatography, Gas; Cross-reactivity; E coli; Escherichia coli - chemistry; Escherichia coli - immunology; Hafnia alvei; Hafnia alvei - chemistry; Hafnia alvei - genetics; Hafnium oxide; Immunoblotting; Klebsiella; Klebsiella pneumoniae - chemistry; Klebsiella pneumoniae - immunology; Lipopolysaccharide; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Mannan; Mannans; Methylation; NMR; NMR spectroscopy; Nuclear magnetic resonance; O Antigens - chemistry; O Antigens - immunology; O Antigens - isolation & purification; Organic chemistry; O‐Specific polysaccharide structure; Polysaccharides; Saccharides; Serological cross‐reactivity; Serology; Two dimensional analysis; α‐d‐Mannan
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1111/j.1574-695X.2001.tb01574.x

Abstract On the basis of chemical and methylation analyses, one- and two-dimensional 1H- and 13C-NMR spectroscopy, including COSY, TOCSY, NOESY and 1H, 13C HSQC experiments, a neutral O-specific polysaccharide isolated from Hafnia alvei strain PCM 1223 lipopolysaccharide (LPS) was found to be an a-mannan composed of pentasaccharide repeating units having the following structure: Immunoblotting showed a strong cross-reactivity between anti-H. alvei PCM 1223 serum and LPSs of Escherichia coli O9 and Klebsiella pneumoniae O3. The serological relationship of the LPSs of these bacteria is due to the structural identity of their O-specific polysaccharides, though the LPSs differ in their core regions.