Investigation of the ternary D-myo-inositol 1,2,6-tris(phosphate)-spermine-Zn2+ system in solution

Interactions of Ins(1,2,6)P3 (IP), with spermine (Spm) and zinc cations have been studied by potentiometric and 31P NMR titrations. In the 4-11 pH range, two IPSpmZn2H3 and IPSpmZn2H mixed complexes are formed which are largely predominant with respect to the binary species. According to 31P NMR tit...

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Bibliographic Details
Published in:Journal of inorganic biochemistry 2001-03, Vol.84 (1-2), p.107-111
Main Authors: Felemez, M, Spiess, B
Format: Article
Language:English
Subjects:
In Vitro Techniques
Inositol Phosphates - chemistry
Magnetic Resonance Spectroscopy
Potentiometry
Second Messenger Systems
Solutions
Spermine - chemistry
Zinc - chemistry
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Summary:Interactions of Ins(1,2,6)P3 (IP), with spermine (Spm) and zinc cations have been studied by potentiometric and 31P NMR titrations. In the 4-11 pH range, two IPSpmZn2H3 and IPSpmZn2H mixed complexes are formed which are largely predominant with respect to the binary species. According to 31P NMR titration it is likely that one of the zinc cations preferably binds phosphates P1 and P6. The adduct formation between Ins(1,2,6)P3 and spermine seems also favourable to the formation of the mixed complexes. The occurrence of ternary complexes involving inositol-phosphates, biogenic amines, and metallic cations may be of relevance in the regulation of biological processes.
ISSN:0162-0134
DOI:10.1016/s0162-0134(00)00220-8