Apoptosis of Endothelial Cell Line ECV304 Persistently Infected with Orientia tsutsugamushi

Endothelial cells are major targets of Orientia tsutsugamushi. To examine the consequences of the infection of endothelial cells with O. tsutsugamushi, we used human endothelial cell line ECV304. Persistent infection was established and infected cultures could be maintained for over seven months wit...

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Published in:MICROBIOLOGY and IMMUNOLOGY 1999, Vol.43(8), pp.751-757
Main Authors: Kim, Mee-kyung, Kee, Sun-ho, Cho, Kyung-A, Chung, Moon-hyun, Lim, Byung-uk, Chang, Woo-hyun, Kang, Jae-seung
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Bacteriology
Biological and medical sciences
Cell Line
Cytokines - biosynthesis
Cytokines - genetics
Endothelium, Vascular - pathology
Fundamental and applied biological sciences. Psychology
Humans
Mice
Microbiology
Orientia tsutsugamushi
Orientia tsutsugamushi - growth & development
Orientia tsutsugamushi - immunology
Orientia tsutsugamushi - physiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Persistent infection
Time Factors
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Summary:Endothelial cells are major targets of Orientia tsutsugamushi. To examine the consequences of the infection of endothelial cells with O. tsutsugamushi, we used human endothelial cell line ECV304. Persistent infection was established and infected cultures could be maintained for over seven months without the addition of normal cells. The heavily infected cells became round and floated in the culture medium, harboring large numbers of organisms inside them. Some of the infected ECV304 cells showed features of apoptotic cells, as determined by the terminal deoxytransferase-mediated dUTP nick end-labeling reaction and DNA fragmentation. We also found that O. tsutsugamushi increased transcription of the mRNAs of proinflammatory cytokines such as IL-6 and IL-8. These results show the first evidence of in vitro-persistent infection by O. tsutsugamushi, which may be related to in vivo persistence reported previously.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.1999.tb02466.x