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Transrectal ultrasound for monitoring murine orthotopic prostate tumor
BACKGROUND The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor...
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| Published in: | The Prostate 2001-05, Vol.47 (2), p.118-124 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4604-bab685dd321db7811cc473f92c257e8d8d89f0f78613866aa22118652b8e2a703 |
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| cites | cdi_FETCH-LOGICAL-c4604-bab685dd321db7811cc473f92c257e8d8d89f0f78613866aa22118652b8e2a703 |
| container_end_page | 124 |
| container_issue | 2 |
| container_start_page | 118 |
| container_title | The Prostate |
| container_volume | 47 |
| creator | Kusaka, Nobuyuki Nasu, Yasutomo Arata, Ryoji Saika, Takashi Tsushima, Tomoyasu Kraaij, Robert Bangma, Chris H. Kumon, Hiromi |
| description | BACKGROUND
The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor growth monitoring. We have applied recent endoluminal ultrasound technology, transrectal ultrasonography (TRUS), to the monitoring of mouse orthotopic prostate tumors.
METHODS
A 6 Fr. 20 MHz catheter‐based radial scan probe was used and TRUS was performed without any prior preparation including anesthesia. Orthotopic tumors were initiated by inoculation of 5000 RM‐9 cells into the dorsal prostate of 12‐week‐old C57BL/6 male mice. The tumor growth was monitored by TRUS from day 3 to day 21. In addition, TRUS was performed to detect tumor growth suppression after intraperitoneal administration of cis‐diamminedichloroplatinum (CDDP).
RESULTS
By ultrasound, tumors became detectable 7 days after tumor cell inoculation. TRUS images were clear and parallel to actual tumor growth. The tumor volume (X) calculated by TRUS correlated significantly with the actual tumor weight (Y) measured at autopsy; Y = 101.653 + 1.174X (R = 0.930, P |
| doi_str_mv | 10.1002/pros.1054 |
| format | article |
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The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor growth monitoring. We have applied recent endoluminal ultrasound technology, transrectal ultrasonography (TRUS), to the monitoring of mouse orthotopic prostate tumors.
METHODS
A 6 Fr. 20 MHz catheter‐based radial scan probe was used and TRUS was performed without any prior preparation including anesthesia. Orthotopic tumors were initiated by inoculation of 5000 RM‐9 cells into the dorsal prostate of 12‐week‐old C57BL/6 male mice. The tumor growth was monitored by TRUS from day 3 to day 21. In addition, TRUS was performed to detect tumor growth suppression after intraperitoneal administration of cis‐diamminedichloroplatinum (CDDP).
RESULTS
By ultrasound, tumors became detectable 7 days after tumor cell inoculation. TRUS images were clear and parallel to actual tumor growth. The tumor volume (X) calculated by TRUS correlated significantly with the actual tumor weight (Y) measured at autopsy; Y = 101.653 + 1.174X (R = 0.930, P < 0.001). Similarly, tumor growth suppression induced by CDDP was clearly detected by TRUS with reasonable accuracy.
CONCLUSIONS
A high resolution TRUS allows simple and reliable monitoring of in situ tumor growth and growth suppression, making the mouse orthotopic prostate tumor model more efficient. Prostate 47:118–124, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.1054</identifier><identifier>PMID: 11340634</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>animal model ; Animals ; Antineoplastic Agents - pharmacology ; Cell Division - drug effects ; Cisplatin - pharmacology ; Disease Models, Animal ; Male ; Mice ; Mice, Inbred C57BL ; Monitoring, Physiologic - methods ; orthotopic implantation ; Prostate - diagnostic imaging ; Prostate - pathology ; prostate cancer ; Prostatic Neoplasms - diagnostic imaging ; Prostatic Neoplasms - drug therapy ; Prostatic Neoplasms - pathology ; Tumor Cells, Cultured ; Ultrasonography</subject><ispartof>The Prostate, 2001-05, Vol.47 (2), p.118-124</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>Copyright 2001 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4604-bab685dd321db7811cc473f92c257e8d8d89f0f78613866aa22118652b8e2a703</citedby><cites>FETCH-LOGICAL-c4604-bab685dd321db7811cc473f92c257e8d8d89f0f78613866aa22118652b8e2a703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11340634$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kusaka, Nobuyuki</creatorcontrib><creatorcontrib>Nasu, Yasutomo</creatorcontrib><creatorcontrib>Arata, Ryoji</creatorcontrib><creatorcontrib>Saika, Takashi</creatorcontrib><creatorcontrib>Tsushima, Tomoyasu</creatorcontrib><creatorcontrib>Kraaij, Robert</creatorcontrib><creatorcontrib>Bangma, Chris H.</creatorcontrib><creatorcontrib>Kumon, Hiromi</creatorcontrib><title>Transrectal ultrasound for monitoring murine orthotopic prostate tumor</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor growth monitoring. We have applied recent endoluminal ultrasound technology, transrectal ultrasonography (TRUS), to the monitoring of mouse orthotopic prostate tumors.
METHODS
A 6 Fr. 20 MHz catheter‐based radial scan probe was used and TRUS was performed without any prior preparation including anesthesia. Orthotopic tumors were initiated by inoculation of 5000 RM‐9 cells into the dorsal prostate of 12‐week‐old C57BL/6 male mice. The tumor growth was monitored by TRUS from day 3 to day 21. In addition, TRUS was performed to detect tumor growth suppression after intraperitoneal administration of cis‐diamminedichloroplatinum (CDDP).
RESULTS
By ultrasound, tumors became detectable 7 days after tumor cell inoculation. TRUS images were clear and parallel to actual tumor growth. The tumor volume (X) calculated by TRUS correlated significantly with the actual tumor weight (Y) measured at autopsy; Y = 101.653 + 1.174X (R = 0.930, P < 0.001). Similarly, tumor growth suppression induced by CDDP was clearly detected by TRUS with reasonable accuracy.
CONCLUSIONS
A high resolution TRUS allows simple and reliable monitoring of in situ tumor growth and growth suppression, making the mouse orthotopic prostate tumor model more efficient. Prostate 47:118–124, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>animal model</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cisplatin - pharmacology</subject><subject>Disease Models, Animal</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Monitoring, Physiologic - methods</subject><subject>orthotopic implantation</subject><subject>Prostate - diagnostic imaging</subject><subject>Prostate - pathology</subject><subject>prostate cancer</subject><subject>Prostatic Neoplasms - diagnostic imaging</subject><subject>Prostatic Neoplasms - drug therapy</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Tumor Cells, Cultured</subject><subject>Ultrasonography</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp1kD9PwzAQxS0EoqUw8AVQJiSG0POfxOmIKC2gikIpMFpO4kAgiYvtCPrtcZUKJnTDu-F37-4eQscYzjEAGa6Mtr6L2A7qYxjxEIBFu6gPhEPIMOU9dGDtO4CngeyjHsaUQUxZH02WRjbWqMzJKmgrZ6TVbZMHhTZBrZvSaVM2r0HdelGBNu5NO70qs2Cz00mnAtfW2hyivUJWVh1tdYCeJlfLy-twNp_eXF7MwozFwMJUpnES5TklOE95gnGWMU6LEclIxFWS-xoVUPAkxjSJYykJwTiJI5ImikgOdIBOO1-__rNV1om6tJmqKtko3VrBISERUOrBsw7M_J3-v0KsTFlLsxYYxCY0sXlAbELz7MnWtE1rlf-R25Q8MOyAr7JS6_-dxP1i_ri1DLuJ0jr1_TshzYeIOeWReLmbiuj5djxmD0uxoD-SrYar</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Kusaka, Nobuyuki</creator><creator>Nasu, Yasutomo</creator><creator>Arata, Ryoji</creator><creator>Saika, Takashi</creator><creator>Tsushima, Tomoyasu</creator><creator>Kraaij, Robert</creator><creator>Bangma, Chris H.</creator><creator>Kumon, Hiromi</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010501</creationdate><title>Transrectal ultrasound for monitoring murine orthotopic prostate tumor</title><author>Kusaka, Nobuyuki ; Nasu, Yasutomo ; Arata, Ryoji ; Saika, Takashi ; Tsushima, Tomoyasu ; Kraaij, Robert ; Bangma, Chris H. ; Kumon, Hiromi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4604-bab685dd321db7811cc473f92c257e8d8d89f0f78613866aa22118652b8e2a703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>animal model</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cisplatin - pharmacology</topic><topic>Disease Models, Animal</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Monitoring, Physiologic - methods</topic><topic>orthotopic implantation</topic><topic>Prostate - diagnostic imaging</topic><topic>Prostate - pathology</topic><topic>prostate cancer</topic><topic>Prostatic Neoplasms - diagnostic imaging</topic><topic>Prostatic Neoplasms - drug therapy</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Tumor Cells, Cultured</topic><topic>Ultrasonography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kusaka, Nobuyuki</creatorcontrib><creatorcontrib>Nasu, Yasutomo</creatorcontrib><creatorcontrib>Arata, Ryoji</creatorcontrib><creatorcontrib>Saika, Takashi</creatorcontrib><creatorcontrib>Tsushima, Tomoyasu</creatorcontrib><creatorcontrib>Kraaij, Robert</creatorcontrib><creatorcontrib>Bangma, Chris H.</creatorcontrib><creatorcontrib>Kumon, Hiromi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kusaka, Nobuyuki</au><au>Nasu, Yasutomo</au><au>Arata, Ryoji</au><au>Saika, Takashi</au><au>Tsushima, Tomoyasu</au><au>Kraaij, Robert</au><au>Bangma, Chris H.</au><au>Kumon, Hiromi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transrectal ultrasound for monitoring murine orthotopic prostate tumor</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>47</volume><issue>2</issue><spage>118</spage><epage>124</epage><pages>118-124</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>BACKGROUND
The mouse orthotopic prostate tumor model has been recognized as an ideal preclinical animal model simulating the anatomical and biological milieu of the prostate. In comparison with the subcutaneous tumor model, the only disadvantage of this model is the difficulty of chronological tumor growth monitoring. We have applied recent endoluminal ultrasound technology, transrectal ultrasonography (TRUS), to the monitoring of mouse orthotopic prostate tumors.
METHODS
A 6 Fr. 20 MHz catheter‐based radial scan probe was used and TRUS was performed without any prior preparation including anesthesia. Orthotopic tumors were initiated by inoculation of 5000 RM‐9 cells into the dorsal prostate of 12‐week‐old C57BL/6 male mice. The tumor growth was monitored by TRUS from day 3 to day 21. In addition, TRUS was performed to detect tumor growth suppression after intraperitoneal administration of cis‐diamminedichloroplatinum (CDDP).
RESULTS
By ultrasound, tumors became detectable 7 days after tumor cell inoculation. TRUS images were clear and parallel to actual tumor growth. The tumor volume (X) calculated by TRUS correlated significantly with the actual tumor weight (Y) measured at autopsy; Y = 101.653 + 1.174X (R = 0.930, P < 0.001). Similarly, tumor growth suppression induced by CDDP was clearly detected by TRUS with reasonable accuracy.
CONCLUSIONS
A high resolution TRUS allows simple and reliable monitoring of in situ tumor growth and growth suppression, making the mouse orthotopic prostate tumor model more efficient. Prostate 47:118–124, 2001. © 2001 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11340634</pmid><doi>10.1002/pros.1054</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Prostate, 2001-05, Vol.47 (2), p.118-124 |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | animal model Animals Antineoplastic Agents - pharmacology Cell Division - drug effects Cisplatin - pharmacology Disease Models, Animal Male Mice Mice, Inbred C57BL Monitoring, Physiologic - methods orthotopic implantation Prostate - diagnostic imaging Prostate - pathology prostate cancer Prostatic Neoplasms - diagnostic imaging Prostatic Neoplasms - drug therapy Prostatic Neoplasms - pathology Tumor Cells, Cultured Ultrasonography |
| title | Transrectal ultrasound for monitoring murine orthotopic prostate tumor |
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