Identification of bloodmeals in haematophagous diptera by cytochrome B heteroduplex analysis

We developed a DNA assay for bloodmeal identification in haematophagous insects. Specific host cytochrome B gene sequences were amplified by PCR and classified on the basis of their mobility in a heteroduplex assay. In the blackfly Simulium damnosum s.l. (Diptera: Simuliidae), human cytochrome B DNA...

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Bibliographic Details
Published in:Medical and veterinary entomology 1999-07, Vol.13 (3), p.282-287
Main Authors: Boakye, D.A, Tang, J, Truc, P, Merriweather, A, Unnasch, T.R
Format: Article
Language:English
Subjects:
Animals
Blood
Cattle
Culex
cytochrome B
cytochrome B gene
Cytochrome b Group - genetics
Female
genetic techniques and protocols
Glossina
Glossina palpalis
Glossinidae
hematophagy
heteroduplex analysis
host identification
hosts
Humans
identification
Ivory Coast
Mice
Nucleic Acid Heteroduplexes
nucleotide sequences
polymerase chain reaction
Sigmodontinae
Simuliidae
Simulium
Simulium damnosum
species identification
structural genes
Swine
Tsetse Flies
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Summary:We developed a DNA assay for bloodmeal identification in haematophagous insects. Specific host cytochrome B gene sequences were amplified by PCR and classified on the basis of their mobility in a heteroduplex assay. In the blackfly Simulium damnosum s.l. (Diptera: Simuliidae), human cytochrome B DNA sequences were identifiable up to 3 days following ingestion of the bloodmeal. In the tsetse Glossina palpalis (Diptera: Glossinidae) collected from tsetse traps in Ivory Coast, bloodmeals were identified as taken from domestic pigs on the basis of their heteroduplex pattern and DNA sequence. Evidently the cytochrome B sequence shows sufficient interspecific variation to distinguish between mammalian host samples, while exhibiting minimal intraspecific variation. The stability of DNA in bloodmeals, for several days post-ingestion by haematophagous insects, allows PCR-HDA assays to be used reliably for host identification.
ISSN:0269-283X
1365-2915
DOI:10.1046/j.1365-2915.1999.00193.x