Pore-forming properties of elicitors of plant defense reactions and cellulolytic enzymes

Using the planar lipid bilayer technique, it is shown that a yeast elicitor as well as several cellulolytic enzymes used in protoplasting plant cells contain components which strongly interact with the bilayers. This results in the appearance of transmembrane ion fluxes which may pass through membra...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1999-10, Vol.459 (2), p.263-266
Main Authors: Klüsener, Birgit, Weiler, Elmar W
Format: Article
Language:English
Subjects:
Cells, Cultured
Cellulase
Cellulase - metabolism
Electrophysiology
Ion channel
Ion Transport
Lipid Bilayers - metabolism
Papaver - metabolism
Pectolyase
Planar lipid bilayer
Plants, Medicinal
Protoplasts - physiology
Saccharomyces cerevisiae - chemistry
Yeast elicitor
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using the planar lipid bilayer technique, it is shown that a yeast elicitor as well as several cellulolytic enzymes used in protoplasting plant cells contain components which strongly interact with the bilayers. This results in the appearance of transmembrane ion fluxes which may pass through membrane defect structures and even large conductance pores with unitary conductances above 400 pS. Since membrane depolarization is an immediate response in the process of defense elicitation in plant cells, elicitors may act directly with the lipid phase of cell membranes, causing depolarizations and thus initiating the process of elicitation. When using enzymatically prepared protoplasts in electrophysiological work, contributions to electrical activity by membrane active constituents originating from the enzymes used must be expected.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)01261-2