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Structural studies on the acidic exopolysaccharide from Haloferax denitrificans ATCC 35960
The structure of a linear, acidic exopolysaccharide isolated from the Archaeon Haloferax denitrificans ATCC 35960 has been determined using NMR spectroscopy. The sugar residues in the repeating unit of the polysaccharide were identified as Gal and GlcA2,3NAc after the assignment of the 1H and 13C re...
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Published in: | Carbohydrate research 1999-06, Vol.319 (1), p.133-140 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The structure of a linear, acidic exopolysaccharide isolated from the Archaeon
Haloferax denitrificans ATCC 35960 has been determined using NMR spectroscopy. The sugar residues in the repeating unit of the polysaccharide were identified as Gal and GlcA2,3NAc after the assignment of the
1H and
13C resonances using COSY, HOHAHA, HMQC and HMQC-TOCSY experiments. The sequence of the residues in the polysaccharide was established from the inter-residue connectivities observed in the HMQC
-NOESY plot. The only sugar released on acid hydrolysis was shown to be
d-Gal by GLC analysis, while the absolute configuration of the acidic sugars was shown to be
d by comparison of the carbon chemical shifts with those of model compounds. Partial acid hydrolysis yielded a tetrasaccharide, terminated by
d-Gal at the reducing end, whose structure confirmed that of the repeating unit of the polysaccharide as →4)-β-
d-Glc
pA2,3NAc-(1→4)-β-
d-Glc
pA2,3NAc-(1→4)-α-
d-Glc
pA2,3NAc-(1→3)-α-
d-Gal
p-(1→, where
d-Glc
pA2,3NAc is 2,3-diacetamido-2,3-dideoxy-
d-glucopyranosiduronic acid. |
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ISSN: | 0008-6215 1873-426X |
DOI: | 10.1016/S0008-6215(99)00111-1 |