Analysis of mustard trypsin inhibitor-2 gene expression in response to developmental or environmental induction

• Published in: Planta 2001-04, Vol.212 (5/6), p.710-717
• Main Authors: De Leo, Francesca, Ceci, Luigi R., Jouanin, Lise, Gallerani, Raffaele
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Arabidopsis; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene expression; Gene Expression Regulation, Plant; Gene induction; Generalities. Genetics. Plant material; Genes; Genes, Reporter; Genetics and breeding of economic plants; Glucuronidase - genetics; Glucuronidase - metabolism; Leaves; Molecular and cellular biology; Molecular genetics; Mustard Plant - drug effects; Mustard Plant - genetics; Mustard Plant - metabolism; Mustards; Plant Leaves - physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; Plants, Genetically Modified; Plants, Medicinal; Promoter Regions, Genetic; Protease inhibitors; Reverse transcriptase polymerase chain reaction; RNA; RNA Splice Sites; Seeds - physiology; Transcription, Genetic; Transcriptional Activation; Transgenic insects; Trypsin Inhibitors - genetics; Trypsin Inhibitors - metabolism
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s004250000474

Transcription analysis of a mustard (Sinapis alba L.) serine proteinase inhibitor gene revealed identical 5′ termini of mRNAs synthesized during seed maturation and chemical or wounding induction. Polyadenylation of mRNAs on multiple or single sites differentiated gene expression, increasing the availability of stable mRNAs during seed maturation compared with chemical and wounding induction. Expression of the β-glucuronidase (GUS)-encoding region of the UidA reporter gene, detected under the control of deleted segments of the region flanking on the 5′ side the mti-2 gene, identified a stretch of about 520 bp essential for gene expression. The presence in this region of two ABRE motifs is relevant for plant response to gene induction. Expression of GUS was detectable under different induction stimuli in several organs such as seedlings and leaves and was active to varying extents in the vascular tissues and meristem.