The Centromeric Sister Chromatid Cohesion Site Directs Mcd1p Binding to Adjacent Sequences

Cohesion between sister chromatids occurs along the length of chromosomes, where it plays essential roles in chromosome segregation. We show here that the centromere, a cis-acting cohesion factor, directs the binding of Mcd1p, a cohesin subunit, to at least 2 kb regions flanking centromeres in a seq...

Full description

Saved in:
Bibliographic Details
Published in:Molecular cell 1999-09, Vol.4 (3), p.445-450
Main Authors: Megee, Paul C, Mistrot, Cathy, Guacci, Vincent, Koshland, Douglas
Format: Article
Language:English
Subjects:
AT Rich Sequence
Binding Sites
Cell Cycle Proteins - metabolism
Centromere - metabolism
Chromatids - metabolism
Chromatin - isolation & purification
Chromosomal Proteins, Non-Histone
Chromosomes, Fungal - metabolism
Cohesins
DNA Nucleotidyltransferases - metabolism
DNA-Binding Proteins
Fungal Proteins - metabolism
Nuclear Proteins - metabolism
Phosphoproteins
Polymerase Chain Reaction
Precipitin Tests
Protein Binding
Recombination, Genetic
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cohesion between sister chromatids occurs along the length of chromosomes, where it plays essential roles in chromosome segregation. We show here that the centromere, a cis-acting cohesion factor, directs the binding of Mcd1p, a cohesin subunit, to at least 2 kb regions flanking centromeres in a sequence-independent manner. The centromere is essential for the maintenance as well as the establishment of this cohesin domain. The efficiency of Mcd1p binding within the cohesin domain is independent of the primary nucleotide sequence of the centromere-flanking DNA but correlates with high A+T DNA content. Thus, the function of centromeres in the cohesion of centromere-proximal regions may be analogous to that of enhancers, nucleating cohesin complex binding over an extended chromosomal domain of A+T-rich DNA.
ISSN:1097-2765
1097-4164
DOI:10.1016/S1097-2765(00)80347-0