Characterization of Astragalus sinicus rhizobia by restriction fragment length polymorphism analysis of chromosomal and nodulation genes regions

Two hundred and four isolates of rhizobia were sampled from root nodules of Astragalus sinicus grown in rice fields of six southern provinces of China. Genotypic diversity was determined by Southern hybridization using nodDBC genes as a probe, restriction fragment length polymorphism (RFLP) analysis...

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Bibliographic Details
Published in:Current microbiology 1999-12, Vol.39 (6), p.358-364
Main Authors: GUO, X.-W, ZHANG, X.-X, ZHANG, Z.-M, LI, F.-D
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Blotting, Southern
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genetic Variation
Genetics
Genomics
Genotypes
Microbiology
Plant Roots - microbiology
Plants - microbiology
Plasmids - genetics
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Rhizobiaceae - genetics
Rhizobiaceae - physiology
Rice fields
RNA, Ribosomal, 16S - genetics
RNA, Ribosomal, 23S - genetics
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Summary:Two hundred and four isolates of rhizobia were sampled from root nodules of Astragalus sinicus grown in rice fields of six southern provinces of China. Genotypic diversity was determined by Southern hybridization using nodDBC genes as a probe, restriction fragment length polymorphism (RFLP) analysis of PCR-amplified 16S-23S rDNA intergenic spacers (IGS), and plasmid profile. Our results show that rhizobia associated with A. sinicus were very diverse, and 10 genotypes were resolved within the previously identified dominant 16S rDNA type. Diversity levels varied greatly between different geographical locations. The same nod gene genotypes were harbored by distinct chromosomal types, suggesting that lateral plasmid transfer occurred during the evolution process.
ISSN:0343-8651
1432-0991
DOI:10.1007/s002849900472