A human homolog of Drosophila warts tumor suppressor, h-warts, localized to mitotic apparatus and specifically phosphorylated during mitosis

We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, inc...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1999-10, Vol.459 (2), p.159-165
Main Authors: Nishiyama, Yasuyuki, Hirota, Toru, Morisaki, Tetsuro, Hara, Toshihiro, Marumoto, Tomotoshi, Iida, Shin-ichi, Makino, Keishi, Yamamoto, Hideyuki, Hiraoka, Takehisa, Kitamura, Nobuo, Saya, Hideyuki
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cdc2
Cell Cycle - physiology
Cell Cycle Proteins - genetics
Cell Cycle Proteins - physiology
Centrosome
Chromosome 6q
Chromosome Mapping
Chromosomes, Human, Pair 6
COS Cells
Drosophila - genetics
Drosophila Proteins
Genes, Tumor Suppressor - physiology
HeLa Cells
Humans
Midbody
Mitosis - physiology
Mitotic spindle
Molecular Sequence Data
Nocodazole
Phosphorylation
Protein Kinases
Protein-Serine-Threonine Kinases - chemistry
Sequence Homology, Amino Acid
Spindle Apparatus - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We identified a human homolog of Drosophila warts tumor suppressor gene, termed h-warts, which was mapped at chromosome 6q24-25.1. The h-warts protein has a serine/threonine kinase domain and is localized to centrosomes in interphase cells. However, it becomes localized to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, in a highly dynamic manner during mitosis. Furthermore, h-warts is specifically phosphorylated in cells at mitotic phase, most likely by Cdc2 kinase. These findings suggest that h-warts functions as a component of the mitotic apparatus and is involved in proper progression of mitosis.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(99)01224-7