The effect of monoclonal antibody and route of immunization on the humoral immune response against Porphyromonas gingivalis

• Published in: Oral microbiology and immunology 2001-06, Vol.16 (3), p.153-162
• Main Authors: Van Tilburg, M. L. J. A., Kozarov, E. V., Progulske-Fox, A., Brady, L. J.
• Format: Article
• Language: English
• Subjects: Administration, Intranasal; Administration, Oral; Animals; Antibodies, Bacterial - administration & dosage; Antibodies, Monoclonal - administration & dosage; Antibody Formation - immunology; antibody-antigen complex; Bacterial Vaccines; Bacteriology; Biological and medical sciences; Dentistry; Enzyme-Linked Immunosorbent Assay; Epitopes - classification; Epitopes - immunology; Fundamental and applied biological sciences. Psychology; humoral immunity; Immunity, Mucosal - immunology; Immunization, Passive; Immunoglobulin A - blood; Immunoglobulin A, Secretory - analysis; Immunoglobulin G - blood; Immunoglobulin G - classification; immunomodulation; Mice; Mice, Inbred BALB C; Microbiology; mucosal immunization; murine; Periodontal Diseases - microbiology; Porphyromonas gingivalis; Porphyromonas gingivalis - classification; Porphyromonas gingivalis - immunology; Statistics, Nonparametric; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
• ISSN: 0902-0055; 1399-302X
• DOI: 10.1034/j.1399-302x.2001.016003153.x

Immunomodulation mediated by exogenous antibodies has been proposed as a vaccine strategy to improve immune protection against pathogenic microorganisms and suggested to contribute to protection following passive immunization. To test whether a monoclonal antibody directed against an adhesion epitope of the periodontal pathogen Porphyromonas gingivalis could influence the humoral immune response following mucosal immunization, BALB/c mice were immunized orally or intranasally with P. gingivalis alone or P. gingivalis coated with monoclonal antibody 61BG1.3. Differences in antigenic specificity of anti–P. gingivalis serum immunoglobulin G (IgG) were demonstrated between groups of mice that received monoclonal antibody–coated P. gingivalis versus those that received P. gingivalis alone by either route of immunization. Binding of monoclonal antibody 61BG1.3 to P. gingivalis prior to immunization did not influence the serum IgG subclass distribution. However, minor differences in subclass distribution were observed between the various routes of mucosal immunization. These results support the hypothesis that specific monoclonal antibody bound to a bacterial vaccine can alter the quality of the humoral immune response to that organism.