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Increased Expression of Arginase II in Human Diabetic Corpus Cavernosum: In Diabetic-Associated Erectile Dysfunction

Nitric oxide (NO) is the principal mediator of penile erection. NO is synthesized by nitric oxide synthase (NOS). It has been well documented that the major causative factor contributing to erectile dysfunction in diabetic patients is the reduction in the amount of NO synthesis in the corpora cavern...

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Published in:Biochemical and biophysical research communications 2001-05, Vol.283 (4), p.923-927
Main Authors: Bivalacqua, Trinity J., Hellstrom, Wayne J.G., Kadowitz, Philip J., Champion, Hunter C.
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Language:English
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description Nitric oxide (NO) is the principal mediator of penile erection. NO is synthesized by nitric oxide synthase (NOS). It has been well documented that the major causative factor contributing to erectile dysfunction in diabetic patients is the reduction in the amount of NO synthesis in the corpora cavernosa of the penis resulting in alterations of normal penile homeostasis. Arginase is an enzyme that shares a common substrate with NOS, thus arginase may downregulate NO production by competing with NOS for this substrate, l-arginine. The purpose of the present study was to compare arginase gene expression, protein levels, and enzyme activity in diabetic human cavernosal tissue. When compared to normal human cavernosal tissue, diabetic corpus cavernosum from humans with erectile dysfunction had higher levels of arginase II protein, gene expression, and enzyme activity. In contrast, gene expression and protein levels of arginase I were not significantly different in diabetic cavernosal tissue when compared to control tissue. The reduced ability of diabetic tissue to convert l-arginine to l-citrulline via nitric oxide synthase was reversed by the selective inhibition of arginase by 2(S)-amino-6-boronohexanoic acid (ABH). These data suggest that the increased expression of arginase II in diabetic cavernosal tissue may contribute to the erectile dysfunction associated with this common disease process and may play a role in other manifestations of diabetic disease in which nitric oxide production is decreased.
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subjects arginase
Arginase - antagonists & inhibitors
Arginase - genetics
Arginase - metabolism
Base Sequence
corpora cavernosa
diabetes
Diabetes Complications
Diabetes Mellitus - enzymology
DNA Primers
erectile dysfunction
Erectile Dysfunction - complications
Erectile Dysfunction - enzymology
Humans
Isoenzymes - antagonists & inhibitors
Isoenzymes - genetics
Isoenzymes - metabolism
Male
nitric oxide
Nitric Oxide Synthase - metabolism
NOS
Penis - blood supply
Penis - enzymology
Reverse Transcriptase Polymerase Chain Reaction
title Increased Expression of Arginase II in Human Diabetic Corpus Cavernosum: In Diabetic-Associated Erectile Dysfunction
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