Loading…
Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues
Foot-and-mouth disease is a highly contagious disease of cloven hooved animals. In cattle, both acute and long-term persistent infections occur. Foot-and-mouth disease virus (FMDV), a picornavirus, has been shown, using virus isolation procedures, to replicate in the pharynx and soft palate of cattl...
Saved in:
Published in: | Virus research 1999-07, Vol.62 (1), p.67-76 |
---|---|
Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3 |
---|---|
cites | cdi_FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3 |
container_end_page | 76 |
container_issue | 1 |
container_start_page | 67 |
container_title | Virus research |
container_volume | 62 |
creator | Prato Murphy, Mariana L. Forsyth, Morag A. Belsham, Graham J. Salt, Jeremy S. |
description | Foot-and-mouth disease is a highly contagious disease of cloven hooved animals. In cattle, both acute and long-term persistent infections occur. Foot-and-mouth disease virus (FMDV), a picornavirus, has been shown, using virus isolation procedures, to replicate in the pharynx and soft palate of cattle. In this study, in situ hybridization has been used to detect FMDV RNA within the cells of tissues removed from infected bovines. A digoxigenin-labelled anti-sense RNA probe was prepared corresponding to a region of the FMDV genome encoding part of the RNA-dependent RNA polymerase (3D). The efficacy and specificity of this probe for in situ hybridisation was determined using virus-infected cells in tissue culture. Strong cytoplasmic staining was only detected in FMDV-infected cells. Various tissue samples were collected from FMDV-infected cattle between 5 and 17 days post-infection. Viral RNA was detected by in situ hybridisation within cells of the soft palate, tonsil and pharynx up to 17 days post-infection. This technique is useful for the study of FMDV localization in cattle both during and after the acute clinical phase of disease and may assist in identifying specific sites of virus persistence. |
doi_str_mv | 10.1016/S0168-1702(99)00050-7 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70846982</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0168170299000507</els_id><sourcerecordid>70846982</sourcerecordid><originalsourceid>FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3</originalsourceid><addsrcrecordid>eNqFkUlLBDEQhYMoOi4_QclJ9NCapLuznGQQNxgUXM4hWzORmY4m6ZHx1xsdFW9eqg71vXrwHgD7GJ1ghOnpQxm8wgyRIyGOEUItqtgaGGHOSMUaQdbB6BfZAtspPReI1oxugi2MWlwTzkdAToJRM_-usg89DB3sQsiV6m01D0OeQuuTU8nBhY9Dgve3Y6iX0Pcw-TzA6VJHb3_Ebz5Py0WHhe8dzD6lwaVdsNGpWXJ733sHPF1ePJ5fV5O7q5vz8aQytSC5EqhtKGkF0rUVNWm55VhYTa3WhHUtpaRprTINw5SQRnRaNdY1DvMO1cKart4Bh6u_LzG8Ft8s5z4ZN5up3oUhSYZ4QwUn_4KY1bh4owK2K9DEkFJ0nXyJfq7iUmIkPyuQXxXIz3ylEPKrAsmK7uDbYNBzZ_-oVpkX4GwFuJLHwrsok_GuN8766EyWNvh_LD4AgAeWMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17318190</pqid></control><display><type>article</type><title>Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues</title><source>ScienceDirect Journals</source><creator>Prato Murphy, Mariana L. ; Forsyth, Morag A. ; Belsham, Graham J. ; Salt, Jeremy S.</creator><creatorcontrib>Prato Murphy, Mariana L. ; Forsyth, Morag A. ; Belsham, Graham J. ; Salt, Jeremy S.</creatorcontrib><description>Foot-and-mouth disease is a highly contagious disease of cloven hooved animals. In cattle, both acute and long-term persistent infections occur. Foot-and-mouth disease virus (FMDV), a picornavirus, has been shown, using virus isolation procedures, to replicate in the pharynx and soft palate of cattle. In this study, in situ hybridization has been used to detect FMDV RNA within the cells of tissues removed from infected bovines. A digoxigenin-labelled anti-sense RNA probe was prepared corresponding to a region of the FMDV genome encoding part of the RNA-dependent RNA polymerase (3D). The efficacy and specificity of this probe for in situ hybridisation was determined using virus-infected cells in tissue culture. Strong cytoplasmic staining was only detected in FMDV-infected cells. Various tissue samples were collected from FMDV-infected cattle between 5 and 17 days post-infection. Viral RNA was detected by in situ hybridisation within cells of the soft palate, tonsil and pharynx up to 17 days post-infection. This technique is useful for the study of FMDV localization in cattle both during and after the acute clinical phase of disease and may assist in identifying specific sites of virus persistence.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/S0168-1702(99)00050-7</identifier><identifier>PMID: 10513288</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Aphthovirus - genetics ; Aphthovirus - isolation & purification ; Cattle ; Cell Line ; Cricetinae ; Digoxigenin ; Foot-and-Mouth Disease - pathology ; Foot-and-Mouth Disease - virology ; Foot-and-mouth disease virus ; Foot-and-mouth disease virus A ; In situ hybridization ; In Situ Hybridization - methods ; Microtomy ; Picornavirus ; RNA probe ; RNA, Viral - isolation & purification ; Virus persistence</subject><ispartof>Virus research, 1999-07, Vol.62 (1), p.67-76</ispartof><rights>1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3</citedby><cites>FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10513288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prato Murphy, Mariana L.</creatorcontrib><creatorcontrib>Forsyth, Morag A.</creatorcontrib><creatorcontrib>Belsham, Graham J.</creatorcontrib><creatorcontrib>Salt, Jeremy S.</creatorcontrib><title>Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>Foot-and-mouth disease is a highly contagious disease of cloven hooved animals. In cattle, both acute and long-term persistent infections occur. Foot-and-mouth disease virus (FMDV), a picornavirus, has been shown, using virus isolation procedures, to replicate in the pharynx and soft palate of cattle. In this study, in situ hybridization has been used to detect FMDV RNA within the cells of tissues removed from infected bovines. A digoxigenin-labelled anti-sense RNA probe was prepared corresponding to a region of the FMDV genome encoding part of the RNA-dependent RNA polymerase (3D). The efficacy and specificity of this probe for in situ hybridisation was determined using virus-infected cells in tissue culture. Strong cytoplasmic staining was only detected in FMDV-infected cells. Various tissue samples were collected from FMDV-infected cattle between 5 and 17 days post-infection. Viral RNA was detected by in situ hybridisation within cells of the soft palate, tonsil and pharynx up to 17 days post-infection. This technique is useful for the study of FMDV localization in cattle both during and after the acute clinical phase of disease and may assist in identifying specific sites of virus persistence.</description><subject>Animals</subject><subject>Aphthovirus - genetics</subject><subject>Aphthovirus - isolation & purification</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>Cricetinae</subject><subject>Digoxigenin</subject><subject>Foot-and-Mouth Disease - pathology</subject><subject>Foot-and-Mouth Disease - virology</subject><subject>Foot-and-mouth disease virus</subject><subject>Foot-and-mouth disease virus A</subject><subject>In situ hybridization</subject><subject>In Situ Hybridization - methods</subject><subject>Microtomy</subject><subject>Picornavirus</subject><subject>RNA probe</subject><subject>RNA, Viral - isolation & purification</subject><subject>Virus persistence</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkUlLBDEQhYMoOi4_QclJ9NCapLuznGQQNxgUXM4hWzORmY4m6ZHx1xsdFW9eqg71vXrwHgD7GJ1ghOnpQxm8wgyRIyGOEUItqtgaGGHOSMUaQdbB6BfZAtspPReI1oxugi2MWlwTzkdAToJRM_-usg89DB3sQsiV6m01D0OeQuuTU8nBhY9Dgve3Y6iX0Pcw-TzA6VJHb3_Ebz5Py0WHhe8dzD6lwaVdsNGpWXJ733sHPF1ePJ5fV5O7q5vz8aQytSC5EqhtKGkF0rUVNWm55VhYTa3WhHUtpaRprTINw5SQRnRaNdY1DvMO1cKart4Bh6u_LzG8Ft8s5z4ZN5up3oUhSYZ4QwUn_4KY1bh4owK2K9DEkFJ0nXyJfq7iUmIkPyuQXxXIz3ylEPKrAsmK7uDbYNBzZ_-oVpkX4GwFuJLHwrsok_GuN8766EyWNvh_LD4AgAeWMg</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Prato Murphy, Mariana L.</creator><creator>Forsyth, Morag A.</creator><creator>Belsham, Graham J.</creator><creator>Salt, Jeremy S.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues</title><author>Prato Murphy, Mariana L. ; Forsyth, Morag A. ; Belsham, Graham J. ; Salt, Jeremy S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Aphthovirus - genetics</topic><topic>Aphthovirus - isolation & purification</topic><topic>Cattle</topic><topic>Cell Line</topic><topic>Cricetinae</topic><topic>Digoxigenin</topic><topic>Foot-and-Mouth Disease - pathology</topic><topic>Foot-and-Mouth Disease - virology</topic><topic>Foot-and-mouth disease virus</topic><topic>Foot-and-mouth disease virus A</topic><topic>In situ hybridization</topic><topic>In Situ Hybridization - methods</topic><topic>Microtomy</topic><topic>Picornavirus</topic><topic>RNA probe</topic><topic>RNA, Viral - isolation & purification</topic><topic>Virus persistence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prato Murphy, Mariana L.</creatorcontrib><creatorcontrib>Forsyth, Morag A.</creatorcontrib><creatorcontrib>Belsham, Graham J.</creatorcontrib><creatorcontrib>Salt, Jeremy S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prato Murphy, Mariana L.</au><au>Forsyth, Morag A.</au><au>Belsham, Graham J.</au><au>Salt, Jeremy S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>62</volume><issue>1</issue><spage>67</spage><epage>76</epage><pages>67-76</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>Foot-and-mouth disease is a highly contagious disease of cloven hooved animals. In cattle, both acute and long-term persistent infections occur. Foot-and-mouth disease virus (FMDV), a picornavirus, has been shown, using virus isolation procedures, to replicate in the pharynx and soft palate of cattle. In this study, in situ hybridization has been used to detect FMDV RNA within the cells of tissues removed from infected bovines. A digoxigenin-labelled anti-sense RNA probe was prepared corresponding to a region of the FMDV genome encoding part of the RNA-dependent RNA polymerase (3D). The efficacy and specificity of this probe for in situ hybridisation was determined using virus-infected cells in tissue culture. Strong cytoplasmic staining was only detected in FMDV-infected cells. Various tissue samples were collected from FMDV-infected cattle between 5 and 17 days post-infection. Viral RNA was detected by in situ hybridisation within cells of the soft palate, tonsil and pharynx up to 17 days post-infection. This technique is useful for the study of FMDV localization in cattle both during and after the acute clinical phase of disease and may assist in identifying specific sites of virus persistence.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10513288</pmid><doi>10.1016/S0168-1702(99)00050-7</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0168-1702 |
ispartof | Virus research, 1999-07, Vol.62 (1), p.67-76 |
issn | 0168-1702 1872-7492 |
language | eng |
recordid | cdi_proquest_miscellaneous_70846982 |
source | ScienceDirect Journals |
subjects | Animals Aphthovirus - genetics Aphthovirus - isolation & purification Cattle Cell Line Cricetinae Digoxigenin Foot-and-Mouth Disease - pathology Foot-and-Mouth Disease - virology Foot-and-mouth disease virus Foot-and-mouth disease virus A In situ hybridization In Situ Hybridization - methods Microtomy Picornavirus RNA probe RNA, Viral - isolation & purification Virus persistence |
title | Localization of foot-and-mouth disease virus RNA by in situ hybridization within bovine tissues |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T22%3A05%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Localization%20of%20foot-and-mouth%20disease%20virus%20RNA%20by%20in%20situ%20hybridization%20within%20bovine%20tissues&rft.jtitle=Virus%20research&rft.au=Prato%20Murphy,%20Mariana%20L.&rft.date=1999-07-01&rft.volume=62&rft.issue=1&rft.spage=67&rft.epage=76&rft.pages=67-76&rft.issn=0168-1702&rft.eissn=1872-7492&rft_id=info:doi/10.1016/S0168-1702(99)00050-7&rft_dat=%3Cproquest_cross%3E70846982%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c392t-905462590b3d93258d819db6dbb27f566245dac47162249fba4de4e18f039dcf3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=17318190&rft_id=info:pmid/10513288&rfr_iscdi=true |