Expression of MUC1 Mucins in the Subserosal Layer Correlates with Postsurgical Prognosis of Pathological Tumor Stage 2 Carcinoma of the Gallbladder

• Published in: Clinical cancer research 2001-05, Vol.7 (5), p.1333-1342
• Main Authors: KAWAMOTO, Toru, SHODA, Junichi, TANAKA, Naomi, TODOROKI, Takeshi, IRIMURA, Tatsuro, MIYAHARA, Naoki, FURUKAWA, Masato, UEDA, Tetsuya, ASANO, Toru, KANO, Masahito, KOIKE, Naoto, FUKAO, Katashi
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Antibodies, Monoclonal - immunology; Biological and medical sciences; Biomarkers, Tumor - biosynthesis; Female; Gallbladder Neoplasms - diagnosis; Gallbladder Neoplasms - metabolism; Gallbladder Neoplasms - mortality; Gallbladder Neoplasms - surgery; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Immunoblotting; Immunohistochemistry; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Middle Aged; Mucin-1 - biosynthesis; Mucin-1 - genetics; Mucin-1 - immunology; Prognosis; Recurrence; Retrospective Studies; RNA, Messenger - biosynthesis; Stromal Cells - metabolism; Survival Analysis; Tumors
• ISSN: 1078-0432; 1557-3265

The overall outcome of pT 2 gallbladder carcinoma has not been favorable. Postsurgical recurrence at distant sites occurs in some cases, although the carcinoma was limited to the gallbladder wall. A high level expression of MUC1 mucins with sialylated carbohydrates (sialylated MUC1 mucins) is correlated with poor survival in intrahepatic bile duct carcinoma. In the present study, immunohistochemistry was performed to determine the expression level of sialylated MUC1 mucins, detected by a monoclonal antibody, MY.1E12, in 31 cases of pT 2 gallbladder carcinoma on which curative resections had been performed and to determine the correlation of the expression level of MY.1E12-reactive-MUC1 mucin with mode of recurrence and postsurgical survival. Immunostainings of the MUC1 mucin were recognized in different types of noncancerous pathological epithelia of the gallbladder except for intestinal metaplasia and cancerous epithelia. Immunohistochemical localization was classified into apical, cytoplasmic, and stromal types based on the predominant cellular distribution of MY.1E12-reactive-MUC1 mucin. In 31 cases of pT 2 carcinoma, the localization was apical type in 64%, cytoplasmic type in 71%, and stromal type in 48% of the cases at the deepest invading sites in the subserosal layer. Distant recurrences, i.e. , peritoneal dissemination in 8 patients and liver metastasis in 3 patients, were seen in 8 (53%) of 15 cases of pT 2 carcinoma that had ≥10% of the cancerous epithelia showing stromal localization of the MUC1 mucin at the deepest invading sites and in 2 (12%) of 16 cases that had