A Gonadotropin-Releasing Hormone (GnRH) Receptor Specific for GnRH II in Primates

Mammalian gonadotropin-releasing hormone (GnRH I) is a hypothalamic decapeptide that stimulates gonadotropic hormone secretion upon interaction with its membrane receptors (type I) on pituitary cells, thereby governing reproductive processes. A second releasing hormone (GnRH II) expressed in mammals...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-04, Vol.282 (4), p.1012-1018
Main Authors: Neill, Jimmy D., Duck, L.Wayne, Sellers, Jeffrey C., Musgrove, Lois C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Cloning, Molecular
COS Cells
gonadotropic hormones
gonadotropin-releasing hormone (GnRH)
Gonadotropin-Releasing Hormone - analogs & derivatives
Gonadotropin-Releasing Hormone - metabolism
human genome
Humans
kidney
Macaca mulatta
Molecular Sequence Data
pituitary gland
receptor
Receptors, LHRH - biosynthesis
Receptors, LHRH - genetics
Receptors, LHRH - physiology
reproduction
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Tissue Distribution
Transfection
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Summary:Mammalian gonadotropin-releasing hormone (GnRH I) is a hypothalamic decapeptide that stimulates gonadotropic hormone secretion upon interaction with its membrane receptors (type I) on pituitary cells, thereby governing reproductive processes. A second releasing hormone (GnRH II) expressed in mammals was shown earlier to be expressed in nonmammals and to have its own receptor. Here we demonstrate that a second receptor (type II) gene is present in the human genome, and report the cloning and characterization of its cDNA from monkeys. The cDNA encodes a G-protein-coupled/7 transmembrane receptor having a C-terminal cytoplasmic tail; it resembles more closely the type II receptors of amphibians and fish (∼55% identity) than it does the type I receptor of humans (∼39%). The GnRH type II receptor proved to be experimentally functional with, and specific for, GnRH II. GnRH receptor type II RNA is expressed ubiquitously in human tissues. This is the first report of a GnRH type II receptor in mammals. Its identification will permit exploration of its role in regulating gonadotropin secretion, female sexual behavior, and tumor cell growth.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4678