UVB IRRADIATION SUPPRESSES CYTOKINE PRODUCTION AND INNATE CELLULAR IMMUNE FUNCTIONS IN MICE

We examined whether ultraviolet-B (UVB) irradiation (6kJ/m2) alters cytokine production and other innate immune reactions by murine peritoneal macrophages and peripheral neutrophils. Along with these experiments, serum IgG levels were also assessed. In addition, using scanning electron microscopy (S...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Pa.), 2001-04, Vol.14 (2), p.104-111
Main Authors: Kasahara, S., Aizawa, K., Okamiya, M., Kazuno, N., Mutoh, S., Fugo, H., Cooper, E.L., Wago, H.
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Enzyme-Linked Immunosorbent Assay
Erythrocytes - immunology
Erythrocytes - radiation effects
Female
Immunoglobulin G - blood
Interleukin-1 - biosynthesis
Interleukin-12 - biosynthesis
Lysosomes - enzymology
Lysosomes - radiation effects
Macrophages, Peritoneal - immunology
Macrophages, Peritoneal - metabolism
Macrophages, Peritoneal - radiation effects
Macrophages, Peritoneal - ultrastructure
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Neutrophils - cytology
Neutrophils - immunology
Neutrophils - metabolism
Neutrophils - radiation effects
Oxygen - metabolism
Phagocytosis - radiation effects
Sheep
Ultraviolet Rays
UVB/IL-12/IL-1α/macrophage/Th1,2
Yeasts - immunology
Yeasts - radiation effects
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Summary:We examined whether ultraviolet-B (UVB) irradiation (6kJ/m2) alters cytokine production and other innate immune reactions by murine peritoneal macrophages and peripheral neutrophils. Along with these experiments, serum IgG levels were also assessed. In addition, using scanning electron microscopy (SEM) we observed macrophages that had been exposed to UVB in vitro. Results showed that UVB irradiation: (1) decreased IL-12 production while increasing IL-1α secretion from macrophages, but had no effect on IL-1α from neutrophils; (2) suppressed phagocytosis of macrophages but not of neutrophils; (3) diminished active oxygen production of macrophages but not of neutrophils; (4) had no effect on serum IgG levels; and (5) caused significant cell destruction of macrophages in vitro. These results suggested: (1) that UVB irradiation could induce characteristic suppression of innate immunity; (2) that innate cellular immunity was more susceptible to the effects of UVB irradiation than humoral immunity.
ISSN:1043-4666
1096-0023
DOI:10.1006/cyto.2001.0849