Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer

We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem...

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Bibliographic Details
Published in:Journal of molecular medicine (Berlin, Germany) Germany), 2001-04, Vol.79 (2-3), p.128-132
Main Authors: HADZIJA, Marijana Popovic, KAPITANOVIC, Sanja, RADOSEVIC, Senka, CACEV, Tamara, MIRT, Mirela, KOVACEVIC, Duje, LUKAC, Josip, HADZIJA, Mirko, SPAVENTI, Radan, PAVELIC, Kresimir
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biological and medical sciences
Colonic Neoplasms - genetics
DNA-Binding Proteins - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genes, Tumor Suppressor
Heterozygote
Humans
Male
Medical sciences
Middle Aged
Smad4 Protein
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Trans-Activators - genetics
Tumors
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Summary:We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorphic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18 (51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors (
ISSN:0946-2716
1432-1440
DOI:10.1007/s001090000179