Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer

We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular medicine (Berlin, Germany) Germany), 2001-04, Vol.79 (2-3), p.128-132
Main Authors: HADZIJA, Marijana Popovic, KAPITANOVIC, Sanja, RADOSEVIC, Senka, CACEV, Tamara, MIRT, Mirela, KOVACEVIC, Duje, LUKAC, Josip, HADZIJA, Mirko, SPAVENTI, Radan, PAVELIC, Kresimir
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biological and medical sciences
Colonic Neoplasms - genetics
DNA-Binding Proteins - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genes, Tumor Suppressor
Heterozygote
Humans
Male
Medical sciences
Middle Aged
Smad4 Protein
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Trans-Activators - genetics
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c233t-cf4a07ee422137abf3c1d16767891dbaf6cd0dcbaafa567a79adbc8159f07d453
cites
container_end_page 132
container_issue 2-3
container_start_page 128
container_title Journal of molecular medicine (Berlin, Germany)
container_volume 79
creator HADZIJA, Marijana Popovic
KAPITANOVIC, Sanja
RADOSEVIC, Senka
CACEV, Tamara
MIRT, Mirela
KOVACEVIC, Duje
LUKAC, Josip
HADZIJA, Mirko
SPAVENTI, Radan
PAVELIC, Kresimir
description We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorphic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18 (51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors (
doi_str_mv 10.1007/s001090000179
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70848804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70848804</sourcerecordid><originalsourceid>FETCH-LOGICAL-c233t-cf4a07ee422137abf3c1d16767891dbaf6cd0dcbaafa567a79adbc8159f07d453</originalsourceid><addsrcrecordid>eNpVkE1LxDAQhoMo7rp69Co5iLdq0qRJc5T1ExYU1HNJ87FbaZuaaQ_rr7fLFtS5zDA8vPA-CJ1Tck0JkTdACCWKjEOlOkBzylmaUM7JIZoTxUWSSipm6ATgc4dkih-jGaUsk4qJOXpbBQAcPN643sXwvV0HqPrt7nP3uuS4H5oQMQxdFx3AeK5d63DV4s3Q6BZDF6K2lcEm1KHFRrfGxVN05HUN7mzaC_TxcP--fEpWL4_Py9tVYlLG-sR4rol0jqcpZVKXnhlqqZBC5oraUnthLLGm1NrrTEgtlbalyWmmPJGWZ2yBrva5XQxfg4O-aCowrq5168IAhSQ5z3PCRzDZgyaObaPzRRerRsdtQUmxs1j8szjyF1PwUDbO_tKTthG4nAANRtc-jr0r-JNKWU4E-wElRnor</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70848804</pqid></control><display><type>article</type><title>Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer</title><source>Springer Link</source><creator>HADZIJA, Marijana Popovic ; KAPITANOVIC, Sanja ; RADOSEVIC, Senka ; CACEV, Tamara ; MIRT, Mirela ; KOVACEVIC, Duje ; LUKAC, Josip ; HADZIJA, Mirko ; SPAVENTI, Radan ; PAVELIC, Kresimir</creator><creatorcontrib>HADZIJA, Marijana Popovic ; KAPITANOVIC, Sanja ; RADOSEVIC, Senka ; CACEV, Tamara ; MIRT, Mirela ; KOVACEVIC, Duje ; LUKAC, Josip ; HADZIJA, Mirko ; SPAVENTI, Radan ; PAVELIC, Kresimir</creatorcontrib><description>We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorphic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18 (51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors (&lt;5 cm) than in larger ones. There was no correlation between DPC4 LOH and age or sex of patients. There was a negative correlation between DPC4 LOH and histological grade or Dukes' stage of tumors, but without statistic significance. Observed results are in agreement with the view that malignant progression is consequence of many genetic changes. It can be concluded that inactivation of the DPC4 gene plays a role in a multistep process of outgrowth and progression of colon cancer.</description><identifier>ISSN: 0946-2716</identifier><identifier>EISSN: 1432-1440</identifier><identifier>DOI: 10.1007/s001090000179</identifier><identifier>PMID: 11357936</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Colonic Neoplasms - genetics ; DNA-Binding Proteins - genetics ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Genes, Tumor Suppressor ; Heterozygote ; Humans ; Male ; Medical sciences ; Middle Aged ; Smad4 Protein ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Trans-Activators - genetics ; Tumors</subject><ispartof>Journal of molecular medicine (Berlin, Germany), 2001-04, Vol.79 (2-3), p.128-132</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c233t-cf4a07ee422137abf3c1d16767891dbaf6cd0dcbaafa567a79adbc8159f07d453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1013806$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11357936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HADZIJA, Marijana Popovic</creatorcontrib><creatorcontrib>KAPITANOVIC, Sanja</creatorcontrib><creatorcontrib>RADOSEVIC, Senka</creatorcontrib><creatorcontrib>CACEV, Tamara</creatorcontrib><creatorcontrib>MIRT, Mirela</creatorcontrib><creatorcontrib>KOVACEVIC, Duje</creatorcontrib><creatorcontrib>LUKAC, Josip</creatorcontrib><creatorcontrib>HADZIJA, Mirko</creatorcontrib><creatorcontrib>SPAVENTI, Radan</creatorcontrib><creatorcontrib>PAVELIC, Kresimir</creatorcontrib><title>Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer</title><title>Journal of molecular medicine (Berlin, Germany)</title><addtitle>J Mol Med (Berl)</addtitle><description>We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorphic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18 (51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors (&lt;5 cm) than in larger ones. There was no correlation between DPC4 LOH and age or sex of patients. There was a negative correlation between DPC4 LOH and histological grade or Dukes' stage of tumors, but without statistic significance. Observed results are in agreement with the view that malignant progression is consequence of many genetic changes. It can be concluded that inactivation of the DPC4 gene plays a role in a multistep process of outgrowth and progression of colon cancer.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Colonic Neoplasms - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Genes, Tumor Suppressor</subject><subject>Heterozygote</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Smad4 Protein</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Trans-Activators - genetics</subject><subject>Tumors</subject><issn>0946-2716</issn><issn>1432-1440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpVkE1LxDAQhoMo7rp69Co5iLdq0qRJc5T1ExYU1HNJ87FbaZuaaQ_rr7fLFtS5zDA8vPA-CJ1Tck0JkTdACCWKjEOlOkBzylmaUM7JIZoTxUWSSipm6ATgc4dkih-jGaUsk4qJOXpbBQAcPN643sXwvV0HqPrt7nP3uuS4H5oQMQxdFx3AeK5d63DV4s3Q6BZDF6K2lcEm1KHFRrfGxVN05HUN7mzaC_TxcP--fEpWL4_Py9tVYlLG-sR4rol0jqcpZVKXnhlqqZBC5oraUnthLLGm1NrrTEgtlbalyWmmPJGWZ2yBrva5XQxfg4O-aCowrq5168IAhSQ5z3PCRzDZgyaObaPzRRerRsdtQUmxs1j8szjyF1PwUDbO_tKTthG4nAANRtc-jr0r-JNKWU4E-wElRnor</recordid><startdate>200104</startdate><enddate>200104</enddate><creator>HADZIJA, Marijana Popovic</creator><creator>KAPITANOVIC, Sanja</creator><creator>RADOSEVIC, Senka</creator><creator>CACEV, Tamara</creator><creator>MIRT, Mirela</creator><creator>KOVACEVIC, Duje</creator><creator>LUKAC, Josip</creator><creator>HADZIJA, Mirko</creator><creator>SPAVENTI, Radan</creator><creator>PAVELIC, Kresimir</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200104</creationdate><title>Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer</title><author>HADZIJA, Marijana Popovic ; KAPITANOVIC, Sanja ; RADOSEVIC, Senka ; CACEV, Tamara ; MIRT, Mirela ; KOVACEVIC, Duje ; LUKAC, Josip ; HADZIJA, Mirko ; SPAVENTI, Radan ; PAVELIC, Kresimir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c233t-cf4a07ee422137abf3c1d16767891dbaf6cd0dcbaafa567a79adbc8159f07d453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Colonic Neoplasms - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Genes, Tumor Suppressor</topic><topic>Heterozygote</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Smad4 Protein</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</topic><topic>Trans-Activators - genetics</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HADZIJA, Marijana Popovic</creatorcontrib><creatorcontrib>KAPITANOVIC, Sanja</creatorcontrib><creatorcontrib>RADOSEVIC, Senka</creatorcontrib><creatorcontrib>CACEV, Tamara</creatorcontrib><creatorcontrib>MIRT, Mirela</creatorcontrib><creatorcontrib>KOVACEVIC, Duje</creatorcontrib><creatorcontrib>LUKAC, Josip</creatorcontrib><creatorcontrib>HADZIJA, Mirko</creatorcontrib><creatorcontrib>SPAVENTI, Radan</creatorcontrib><creatorcontrib>PAVELIC, Kresimir</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular medicine (Berlin, Germany)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HADZIJA, Marijana Popovic</au><au>KAPITANOVIC, Sanja</au><au>RADOSEVIC, Senka</au><au>CACEV, Tamara</au><au>MIRT, Mirela</au><au>KOVACEVIC, Duje</au><au>LUKAC, Josip</au><au>HADZIJA, Mirko</au><au>SPAVENTI, Radan</au><au>PAVELIC, Kresimir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer</atitle><jtitle>Journal of molecular medicine (Berlin, Germany)</jtitle><addtitle>J Mol Med (Berl)</addtitle><date>2001-04</date><risdate>2001</risdate><volume>79</volume><issue>2-3</issue><spage>128</spage><epage>132</epage><pages>128-132</pages><issn>0946-2716</issn><eissn>1432-1440</eissn><abstract>We investigated the prevalence of DPC4 loss of heterozygosity in sporadic colorectal cancer. Thirty-six cases of human sporadic colon carcinoma and corresponding normal tissue samples were examined to evaluate loss of heterozygosity at the DPC4 tumor suppressor locus using variable nucleotide tandem repeat (VNTR) analysis and three polymorphic markers. From 36 analyzed samples 35 (97%) were heterozygous or informative. Loss of heterozygosity at the DPC4 locus was detected in 18 (51%) of informative tumor DNAs. The DPC4 LOH was more frequent in smaller tumors (&lt;5 cm) than in larger ones. There was no correlation between DPC4 LOH and age or sex of patients. There was a negative correlation between DPC4 LOH and histological grade or Dukes' stage of tumors, but without statistic significance. Observed results are in agreement with the view that malignant progression is consequence of many genetic changes. It can be concluded that inactivation of the DPC4 gene plays a role in a multistep process of outgrowth and progression of colon cancer.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>11357936</pmid><doi>10.1007/s001090000179</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0946-2716
ispartof Journal of molecular medicine (Berlin, Germany), 2001-04, Vol.79 (2-3), p.128-132
issn 0946-2716
1432-1440
language eng
recordid cdi_proquest_miscellaneous_70848804
source Springer Link
subjects Aged
Aged, 80 and over
Biological and medical sciences
Colonic Neoplasms - genetics
DNA-Binding Proteins - genetics
Female
Gastroenterology. Liver. Pancreas. Abdomen
Genes, Tumor Suppressor
Heterozygote
Humans
Male
Medical sciences
Middle Aged
Smad4 Protein
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Trans-Activators - genetics
Tumors
title Loss of heterozygosity of DPC4 tumor suppressor gene in human sporadic colon cancer
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T14%3A48%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Loss%20of%20heterozygosity%20of%20DPC4%20tumor%20suppressor%20gene%20in%20human%20sporadic%20colon%20cancer&rft.jtitle=Journal%20of%20molecular%20medicine%20(Berlin,%20Germany)&rft.au=HADZIJA,%20Marijana%20Popovic&rft.date=2001-04&rft.volume=79&rft.issue=2-3&rft.spage=128&rft.epage=132&rft.pages=128-132&rft.issn=0946-2716&rft.eissn=1432-1440&rft_id=info:doi/10.1007/s001090000179&rft_dat=%3Cproquest_cross%3E70848804%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c233t-cf4a07ee422137abf3c1d16767891dbaf6cd0dcbaafa567a79adbc8159f07d453%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=70848804&rft_id=info:pmid/11357936&rfr_iscdi=true