Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high-performance liquid chromatography

A HPLC method was developed for the separation and determination of flavonoid and phenolic antioxidants in cranberry juices. Free flavonoid and phenolic compounds were fractionated into neutral and acidic groups by means of a solid-phase extraction method, followed by subsequent HPLC separations. Co...

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Bibliographic Details
Published in:Journal of Chromatography A 2001-04, Vol.913 (1), p.387-395
Main Authors: Chen, Hao, Zuo, Yuegang, Deng, Yiwei
Format: Article
Language:English
Subjects:
Antioxidants
Benzoic acid
Biological and medical sciences
Catechin
Chromatography, High Pressure Liquid - methods
Flavonoids
Flavonoids - analysis
Flavonoids - isolation & purification
Food industries
Fruit - chemistry
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Myricetin
Phenolic acids
Phenols - analysis
Phenols - isolation & purification
Quercetin
Reference Standards
Spectrophotometry, Ultraviolet
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Summary:A HPLC method was developed for the separation and determination of flavonoid and phenolic antioxidants in cranberry juices. Free flavonoid and phenolic compounds were fractionated into neutral and acidic groups by means of a solid-phase extraction method, followed by subsequent HPLC separations. Combined flavonoids and phenolics were hydrolyzed by acid before HPLC analysis. This developed method provides a fast and high resolution of individual flavonoid and phenolic compounds. In cranberry fruit, flavonoids and phenolic acids exist predominantly in combined forms, such as glycosides and esters. A total of 400 mg of total flavonoids and phenolic compounds/l of sample was found in a freshly squeezed cranberry juice, which was distributed as about 44% of phenolic acids and 56% of flavonoids. Benzoic acid was the major phenolic compound. Major flavonoids in the freshly squeezed cranberry juice were quercetin and myricetin.
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)01030-X