Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–T...

Full description

Saved in:
Bibliographic Details
Published in:Journal of magnetic resonance (1997) 1999-11, Vol.141 (1), p.180-184
Main Authors: Loria, J.Patrick, Rance, Mark, Palmer, Arthur G.
Format: Article
Language:English
Subjects:
gradient coherence selection
Magnetic Resonance Spectroscopy - methods
triosephosphate isomerase
triple-resonance NMR spectroscopy
TROSY
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a 15N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer.
ISSN:1090-7807
1096-0856
DOI:10.1006/jmre.1999.1891