Jaagsiekte sheep retrovirus can be detected in the peripheral blood during the pre-clinical period of sheep pulmonary adenomatosis

Peripheral blood leukocytes (PBLs) and tissue samples from 36 sheep were examined for jaagsiekte sheep retrovirus (JSRV) by hemi-nested PCR. Animals were classified according to the status of sheep pulmonary adenomatosis (SPA), which was confirmed by pathological examination, as follows: (i) sheep w...

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Bibliographic Details
Published in:Journal of general virology 2001-06, Vol.82 (6), p.1355-1358
Main Authors: Gonzalez, L, Garcia-Goti, M, Cousens, C, Dewar, P, Cortabarria, N, Extramiana, A.B, Ortin, A, Heras, M. de las, Sharp, J.M
Format: Article
Language:English
Subjects:
adenomatosis
Animals
Disease Progression
DNA
DNA, Viral - blood
flocks
Jaagsiekte sheep retrovirus
Jaagsiekte sheep retrovirus - genetics
Jaagsiekte sheep retrovirus - isolation & purification
leukocytes
Leukocytes - virology
lung neoplasms
Lung Neoplasms - pathology
Lung Neoplasms - veterinary
Lung Neoplasms - virology
Lymphoid Tissue - virology
Polymerase Chain Reaction
Proviruses - genetics
Proviruses - isolation & purification
Pulmonary Adenomatosis, Ovine - blood
Pulmonary Adenomatosis, Ovine - pathology
Pulmonary Adenomatosis, Ovine - virology
Retrovirus
sheep
Sheep - blood
Sheep - virology
sheep pulmonary adenomatosis
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Summary:Peripheral blood leukocytes (PBLs) and tissue samples from 36 sheep were examined for jaagsiekte sheep retrovirus (JSRV) by hemi-nested PCR. Animals were classified according to the status of sheep pulmonary adenomatosis (SPA), which was confirmed by pathological examination, as follows: (i) sheep with classical SPA (cSPA, n = 10), (ii) sheep with atypical SPA (aSPA, n = 6), (iii) nonaffected sheep from SPA-affected flocks (in-contact, n = 10) and (iv) non-affected sheep from SPA-free flocks (control, n = 10). JSRV proviral DNA was detected in the PBLs of 10/10 cSPA, 5/6 aSPA, 4/10 in-contact and 0/10 control sheep. Lung tumours and lymphoid organs were also found to be JSRV-positive. The number of positive PCR results was greater for sheep in the cSPA group than for those in the aSPA and in-contact groups. For the first time, it is concluded that JSRV can be detected in naturally infected sheep before the onset of clinical disease and even before the development of discernible tumours.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-82-6-1355