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Incubation Temperature Influences Sex-Steroid Levels in Juvenile Red-Eared Slider Turtles, Trachemys scripta, a Species with Temperature-Dependent Sex Determination
Incubation temperature determines gonadal sex in the red-eared slider turtle, Trachemys scripta. However, little is known about the long-term effects of incubation temperature on traits other than gonadal sex in this species. To investigate the hypothesis that incubation temperature (independent of...
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| Published in: | Biology of reproduction 1999-11, Vol.61 (5), p.1275-1280 |
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| Language: | English |
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| container_end_page | 1280 |
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| container_title | Biology of reproduction |
| container_volume | 61 |
| creator | Rhen, T Willingham, E Sakata, J T Crews, D |
| description | Incubation temperature determines gonadal sex in the red-eared slider turtle, Trachemys scripta. However, little is known about the long-term effects of incubation temperature on traits other than gonadal sex in this species.
To investigate the hypothesis that incubation temperature (independent of gonadal sex) influences sex steroid levels after
hatching, we incubated eggs of the red-eared slider turtle at three temperatures (26, 28.6, and 31°C). We then measured plasma
levels of dihydrotestosterone, estradiol, progesterone, and testosterone in 6-wk-old males from 26°C and 28.6°C eggs, and
in 6-wk-old females from 28.6°C and 31°C eggs. We found that dihydrotestosterone levels were not influenced by incubation
temperature or gonadal sex. However, progesterone levels were significantly higher in males from 26°C eggs than in males from
28.6°C eggs. In contrast, testosterone levels did not differ between males from 26°C versus males from 28.6°C eggs, but they
were significantly higher in females from 28.6°C than in females from 31°C eggs. Progesterone and testosterone levels did
not differ between males and females from 28.6°C eggs. Temperature also influenced estradiol levels in both sexes, but the
effects were enigmatic. We conclude that incubation temperature has lasting effects on sex steroid levels even after hatching. |
| doi_str_mv | 10.1095/biolreprod61.5.1275 |
| format | article |
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To investigate the hypothesis that incubation temperature (independent of gonadal sex) influences sex steroid levels after
hatching, we incubated eggs of the red-eared slider turtle at three temperatures (26, 28.6, and 31°C). We then measured plasma
levels of dihydrotestosterone, estradiol, progesterone, and testosterone in 6-wk-old males from 26°C and 28.6°C eggs, and
in 6-wk-old females from 28.6°C and 31°C eggs. We found that dihydrotestosterone levels were not influenced by incubation
temperature or gonadal sex. However, progesterone levels were significantly higher in males from 26°C eggs than in males from
28.6°C eggs. In contrast, testosterone levels did not differ between males from 26°C versus males from 28.6°C eggs, but they
were significantly higher in females from 28.6°C than in females from 31°C eggs. Progesterone and testosterone levels did
not differ between males and females from 28.6°C eggs. Temperature also influenced estradiol levels in both sexes, but the
effects were enigmatic. We conclude that incubation temperature has lasting effects on sex steroid levels even after hatching.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod61.5.1275</identifier><identifier>PMID: 10529274</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Dihydrotestosterone - metabolism ; Embryo, Nonmammalian - metabolism ; Female ; Follicle Stimulating Hormone - pharmacology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Gonadal Steroid Hormones - metabolism ; Male ; Non mammalian vertebrate reproduction ; Radioimmunoassay ; Sex Differentiation - physiology ; Temperature ; Testosterone - metabolism ; Trachemys scripta ; Turtles - physiology ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 1999-11, Vol.61 (5), p.1275-1280</ispartof><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1190850$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10529274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rhen, T</creatorcontrib><creatorcontrib>Willingham, E</creatorcontrib><creatorcontrib>Sakata, J T</creatorcontrib><creatorcontrib>Crews, D</creatorcontrib><title>Incubation Temperature Influences Sex-Steroid Levels in Juvenile Red-Eared Slider Turtles, Trachemys scripta, a Species with Temperature-Dependent Sex Determination</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Incubation temperature determines gonadal sex in the red-eared slider turtle, Trachemys scripta. However, little is known about the long-term effects of incubation temperature on traits other than gonadal sex in this species.
To investigate the hypothesis that incubation temperature (independent of gonadal sex) influences sex steroid levels after
hatching, we incubated eggs of the red-eared slider turtle at three temperatures (26, 28.6, and 31°C). We then measured plasma
levels of dihydrotestosterone, estradiol, progesterone, and testosterone in 6-wk-old males from 26°C and 28.6°C eggs, and
in 6-wk-old females from 28.6°C and 31°C eggs. We found that dihydrotestosterone levels were not influenced by incubation
temperature or gonadal sex. However, progesterone levels were significantly higher in males from 26°C eggs than in males from
28.6°C eggs. In contrast, testosterone levels did not differ between males from 26°C versus males from 28.6°C eggs, but they
were significantly higher in females from 28.6°C than in females from 31°C eggs. Progesterone and testosterone levels did
not differ between males and females from 28.6°C eggs. Temperature also influenced estradiol levels in both sexes, but the
effects were enigmatic. We conclude that incubation temperature has lasting effects on sex steroid levels even after hatching.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Dihydrotestosterone - metabolism</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - pharmacology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonadal Steroid Hormones - metabolism</subject><subject>Male</subject><subject>Non mammalian vertebrate reproduction</subject><subject>Radioimmunoassay</subject><subject>Sex Differentiation - physiology</subject><subject>Temperature</subject><subject>Testosterone - metabolism</subject><subject>Trachemys scripta</subject><subject>Turtles - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkstu1DAUhi0EokPhCZCQF6irZvAlceIlagsMGgmJGdaRLyfEyHGC7TT0fXhQUhhUdqzO5tP_HZ3zI_SSki0lsnqj3egjTHG0gm6rLWV19QhtaMVkUTPRPEYbQogoOBf8DD1L6RshtOSMP0VnlKwUq8sN-rkLZtYquzHgIwwTRJXnCHgXOj9DMJDwAX4UhwxxdBbv4RZ8wi7gj_MtBOcBfwZb3KgIFh-8sxDxcY7ZQ7rEx6hMD8NdwslEN2V1iRU-TGDcmrq43P9rLK5hgmAh5HshvobVOLjwe7Pn6EmnfIIXp3mOvry7OV59KPaf3u-u3u6LnlGeC9kZ0-iuhqaUWgqra6o1tY0QsiO1KUvTcFp10lgtmGwEL0HwhgFlUoCUgp-jiz-561G_z5ByO7hkwHsVYJxTW5Om4qyS_wVpXRIqWbWCr07grAew7RTdoOJd-_cBK_D6BKhklO-iCsalB47K1UkehL372i8uQpsG5f2ayttlWQRtq_a-APwXanem0w</recordid><startdate>199911</startdate><enddate>199911</enddate><creator>Rhen, T</creator><creator>Willingham, E</creator><creator>Sakata, J T</creator><creator>Crews, D</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>199911</creationdate><title>Incubation Temperature Influences Sex-Steroid Levels in Juvenile Red-Eared Slider Turtles, Trachemys scripta, a Species with Temperature-Dependent Sex Determination</title><author>Rhen, T ; Willingham, E ; Sakata, J T ; Crews, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h213t-9fcc8bf7e849b96db71bb1d8669f07c44c8315f9cdb6298634e6382e1296e9963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Dihydrotestosterone - metabolism</topic><topic>Embryo, Nonmammalian - metabolism</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - pharmacology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonadal Steroid Hormones - metabolism</topic><topic>Male</topic><topic>Non mammalian vertebrate reproduction</topic><topic>Radioimmunoassay</topic><topic>Sex Differentiation - physiology</topic><topic>Temperature</topic><topic>Testosterone - metabolism</topic><topic>Trachemys scripta</topic><topic>Turtles - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rhen, T</creatorcontrib><creatorcontrib>Willingham, E</creatorcontrib><creatorcontrib>Sakata, J T</creatorcontrib><creatorcontrib>Crews, D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rhen, T</au><au>Willingham, E</au><au>Sakata, J T</au><au>Crews, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Incubation Temperature Influences Sex-Steroid Levels in Juvenile Red-Eared Slider Turtles, Trachemys scripta, a Species with Temperature-Dependent Sex Determination</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1999-11</date><risdate>1999</risdate><volume>61</volume><issue>5</issue><spage>1275</spage><epage>1280</epage><pages>1275-1280</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Incubation temperature determines gonadal sex in the red-eared slider turtle, Trachemys scripta. However, little is known about the long-term effects of incubation temperature on traits other than gonadal sex in this species.
To investigate the hypothesis that incubation temperature (independent of gonadal sex) influences sex steroid levels after
hatching, we incubated eggs of the red-eared slider turtle at three temperatures (26, 28.6, and 31°C). We then measured plasma
levels of dihydrotestosterone, estradiol, progesterone, and testosterone in 6-wk-old males from 26°C and 28.6°C eggs, and
in 6-wk-old females from 28.6°C and 31°C eggs. We found that dihydrotestosterone levels were not influenced by incubation
temperature or gonadal sex. However, progesterone levels were significantly higher in males from 26°C eggs than in males from
28.6°C eggs. In contrast, testosterone levels did not differ between males from 26°C versus males from 28.6°C eggs, but they
were significantly higher in females from 28.6°C than in females from 31°C eggs. Progesterone and testosterone levels did
not differ between males and females from 28.6°C eggs. Temperature also influenced estradiol levels in both sexes, but the
effects were enigmatic. We conclude that incubation temperature has lasting effects on sex steroid levels even after hatching.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>10529274</pmid><doi>10.1095/biolreprod61.5.1275</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biology of reproduction, 1999-11, Vol.61 (5), p.1275-1280 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70853259 |
| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Dihydrotestosterone - metabolism Embryo, Nonmammalian - metabolism Female Follicle Stimulating Hormone - pharmacology Freshwater Fundamental and applied biological sciences. Psychology Gonadal Steroid Hormones - metabolism Male Non mammalian vertebrate reproduction Radioimmunoassay Sex Differentiation - physiology Temperature Testosterone - metabolism Trachemys scripta Turtles - physiology Vertebrates: reproduction |
| title | Incubation Temperature Influences Sex-Steroid Levels in Juvenile Red-Eared Slider Turtles, Trachemys scripta, a Species with Temperature-Dependent Sex Determination |
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