Comparison of Competitively Primed and Conventional Allele-Specific Nucleic Acid Amplification

A simulation of competitively primed allele-specific DNA amplification has been constructed and its behavior examined. This has shown that when the ratio of the amount of homoduplex misprime product to the total amount of amplimer is low, it increases by approximately one-fourth of the mispriming fr...

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Bibliographic Details
Published in:Analytical biochemistry 2001-05, Vol.292 (2), p.207-215
Main Authors: Giffard, P.M., McMahon, J.A., Gustafson, H.M., Barnard, R.T., Voisey, J.
Format: Article
Language:English
Subjects:
Alleles
Binding, Competitive
Computer Simulation
DNA Primers - genetics
Models, Genetic
Polymerase Chain Reaction - methods
Reproducibility of Results
Sensitivity and Specificity
Substrate Specificity
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Summary:A simulation of competitively primed allele-specific DNA amplification has been constructed and its behavior examined. This has shown that when the ratio of the amount of homoduplex misprime product to the total amount of amplimer is low, it increases by approximately one-fourth of the mispriming frequency with each doubling of the total amount of amplimer. When the ratio is high and reverse mispriming becomes significant, it asymptotes toward a value
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.2001.5077