Humoral opsonins of the tunicate, Pyura stolonifera

This study characterizes humoral opsonins from the tunicate, Pyura stolonifera. The predominant opsonic components in P. stolonifera hemolymph were found to be calcium-dependent lectins with broad carbohydrate specificities. The opsonic lectins were purified by carbohydrate affinity chromatography w...

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Bibliographic Details
Published in:Developmental and comparative immunology 2001-06, Vol.25 (5), p.377-385
Main Authors: Pearce, Sarina, Newton, Rebecca A., Nair, Sham V., Raftos, David A.
Format: Article
Language:English
Subjects:
Animals
Ascidian
Carbohydrates
Chromatography, Affinity
Complement
Edetic Acid
Flow Cytometry
Glycoproteins - metabolism
Lectin
Lectins - isolation & purification
Lectins - metabolism
Opsonin Proteins - isolation & purification
Opsonin Proteins - metabolism
Opsonization
Phagocytes
Phagocytosis
Protein Binding
Pyura stolonifera
Tunicate
Urochordata - metabolism
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Summary:This study characterizes humoral opsonins from the tunicate, Pyura stolonifera. The predominant opsonic components in P. stolonifera hemolymph were found to be calcium-dependent lectins with broad carbohydrate specificities. The opsonic lectins were purified by carbohydrate affinity chromatography which eluted a complex pattern of proteins ranging in molecular mass from 80 to >200 kDa. Reducing and two dimensional SDS–PAGE indicated that the diversity of mature lectins evident under non-reducing conditions resulted from the differential oligomerization of two polypeptide sub-units (35 and 22 kDa). In addition to lectin-mediated opsonic activity, hemolymph was also found to contain proteolytically activated opsonins. These data suggest that multiple, possibly interactive opsonic systems co-exist in P. stolonifera.
ISSN:0145-305X
1879-0089
DOI:10.1016/S0145-305X(01)00011-8