An enhanced microfluidic chip coupled to an electrospray Qstar mass spectrometer for protein identification

• Published in: Electrophoresis 2000-01, Vol.21 (1), p.181-190
• Main Authors: Pinto, Devanand M., Ning, Yuebin, Figeys, Daniel
• Format: Article
• Language: English
• Subjects: Animals; Humans; Mass Spectrometry - instrumentation; Mass Spectrometry - methods; Microfluidics; Nonaqueous electrolyte; Polybrene; Protein digests; Proteins - analysis; Proteins - chemistry; Proteomics
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/(SICI)1522-2683(20000101)21:1<181::AID-ELPS181>3.0.CO;2-Q

The combination of microfabricated fluidic systems (μFAB) and electrospray mass spectrometers (ESI‐MS) will provide multiplexed and integrated analytical systems for proteins and other biomolecules. Implementation of this novel approach requires the development of robust and user‐friendly μFAB devices. Here, we present new approaches that improve the robustness, user friendliness and performance of μFAB devices coupled to MS. First, we present the development of a convenient mount to connect a μFAB device to the ESI‐MS and the incorporation of filters in the reservoirs and exit of the μFAB. This mount facilitates interfacing and significantly reduces the chemical noise observed by the MS. Furthermore, we demonstrate improvements in sample handling and delivery by using either a nonaqueous electrolyte or a cationic coating on the surfaces in the μFAB device and transfer capillary. These improvements are applied to protein analysis by continuous infusion of proteolytic digests.