Loading…

Glycolipids Support E-Selectin-Specific Strong Cell Tethering under Flow

This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over α2,3-sialyl Lewis X (α2,3-sLex) presented as the hexaosylceramide glycosphing...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-06, Vol.284 (1), p.42-49
Main Authors: Burdick, Monica M., Bochner, Bruce S., Collins, Brian E., Schnaar, Ronald L., Konstantopoulos, Konstantinos
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over α2,3-sialyl Lewis X (α2,3-sLex) presented as the hexaosylceramide glycosphingolipid adsorbed in a monolayer containing phosphatidylcholine and cholesterol. CHO-E cells tethered extensively and formed slow, stable rolling interactions with α2,3-sLex glycosphingolipid but not with the comparable α2,6-sLex glycosphingolipid. Tethering/rolling varied with wall shear stress, selectin density, and ligand density. In contrast, α2,3-sLex glycosphingolipid supported only limited, fast CHO-P cell rolling. As calculated from a stochastic model of cell rolling, the step size between successive bond releases from the α2,3-sLex glycosphingolipid was smaller for CHO-E than CHO-P cells, whereas the opposite effect was observed for the waiting time between these events. Detachment assays revealed stronger adhesive interactions of CHO-E than CHO-P cells with α2,3-sLex glycosphingolipid. These findings indicate that glycosphingolipids expressing an appropriate oligosaccharide mediate cell tethering/rolling via E-selectin but not P-selectin.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.4899