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Glycolipids Support E-Selectin-Specific Strong Cell Tethering under Flow
This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over α2,3-sialyl Lewis X (α2,3-sLex) presented as the hexaosylceramide glycosphing...
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Published in: | Biochemical and biophysical research communications 2001-06, Vol.284 (1), p.42-49 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | This study provides functional evidence that glycosphingolipids constitute ligands for E-selectin but not P-selectin. Chinese hamster ovary (CHO) cells expressing E-selectin (CHO-E) or P-selectin (CHO-P) were perfused over α2,3-sialyl Lewis X (α2,3-sLex) presented as the hexaosylceramide glycosphingolipid adsorbed in a monolayer containing phosphatidylcholine and cholesterol. CHO-E cells tethered extensively and formed slow, stable rolling interactions with α2,3-sLex glycosphingolipid but not with the comparable α2,6-sLex glycosphingolipid. Tethering/rolling varied with wall shear stress, selectin density, and ligand density. In contrast, α2,3-sLex glycosphingolipid supported only limited, fast CHO-P cell rolling. As calculated from a stochastic model of cell rolling, the step size between successive bond releases from the α2,3-sLex glycosphingolipid was smaller for CHO-E than CHO-P cells, whereas the opposite effect was observed for the waiting time between these events. Detachment assays revealed stronger adhesive interactions of CHO-E than CHO-P cells with α2,3-sLex glycosphingolipid. These findings indicate that glycosphingolipids expressing an appropriate oligosaccharide mediate cell tethering/rolling via E-selectin but not P-selectin. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1006/bbrc.2001.4899 |