Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1

Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approxi...

Full description

Saved in:
Bibliographic Details
Published in:Journal of bioscience and bioengineering 1999, Vol.87 (3), p.383-385
Main Authors: Akiyama, Kouichi, Fujita, Tadashi, Kuroshima, Ken-Ichi, Sakane, Takeshi, Yokota, Akira, Takata, Renkichi
Format: Article
Language:English
Subjects:
Bacillus ehimensis
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
chitosanase
cloning
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983
cites cdi_FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983
container_end_page 385
container_issue 3
container_start_page 383
container_title Journal of bioscience and bioengineering
container_volume 87
creator Akiyama, Kouichi
Fujita, Tadashi
Kuroshima, Ken-Ichi
Sakane, Takeshi
Yokota, Akira
Takata, Renkichi
description Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000. A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.
doi_str_mv 10.1016/S1389-1723(99)80050-4
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70867196</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1389172399800504</els_id><sourcerecordid>70867196</sourcerecordid><originalsourceid>FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983</originalsourceid><addsrcrecordid>eNqFkEtv1TAQRi0Eog_6E1p5gVC7CHhix48VKlUfiEogtXvLccatUWIXO0Hi33Nvb6Quu5pZnG_m0yHkGNhnYCC_3AHXpgHV8lNjzjRjHWvEG7IPXKhGiBbebvcV2SMHtf5mDBRT8J7sgWx5K7TcJz9-LSWG6N0cc6IuDfQBE1I_5hTTA82BOuof45yrS64iDSVP9JvzcRyXSvExTphqrPTy_Bo-kHfBjRWP1nlI7q8u7y9umtuf198vzm8bLzTMjYJOa240ih76Dp3U0pmh4wYCYouehx57YYTkvTKtGPgw6A57HVyH0mh-SD7tzj6V_GfBOtspVo_j6BLmpVrFtFRg5AbsdqAvudaCwT6VOLnyzwKzW4n2WaLdGrLG2GeJVmxyJ-uDpZ9weEmt1jbAxxVw1bsxFJd8rC-cBqPVtujXHYYbGX8jFlt9xORxiAX9bIccX2nyH0kEjZI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70867196</pqid></control><display><type>article</type><title>Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1</title><source>ScienceDirect Journals</source><creator>Akiyama, Kouichi ; Fujita, Tadashi ; Kuroshima, Ken-Ichi ; Sakane, Takeshi ; Yokota, Akira ; Takata, Renkichi</creator><creatorcontrib>Akiyama, Kouichi ; Fujita, Tadashi ; Kuroshima, Ken-Ichi ; Sakane, Takeshi ; Yokota, Akira ; Takata, Renkichi</creatorcontrib><description>Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000. A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/S1389-1723(99)80050-4</identifier><identifier>PMID: 16232486</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>Bacillus ehimensis ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; chitosanase ; cloning ; Fundamental and applied biological sciences. Psychology ; Miscellaneous ; Mission oriented research ; purification</subject><ispartof>Journal of bioscience and bioengineering, 1999, Vol.87 (3), p.383-385</ispartof><rights>1999</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983</citedby><cites>FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1819878$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16232486$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akiyama, Kouichi</creatorcontrib><creatorcontrib>Fujita, Tadashi</creatorcontrib><creatorcontrib>Kuroshima, Ken-Ichi</creatorcontrib><creatorcontrib>Sakane, Takeshi</creatorcontrib><creatorcontrib>Yokota, Akira</creatorcontrib><creatorcontrib>Takata, Renkichi</creatorcontrib><title>Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000. A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.</description><subject>Bacillus ehimensis</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>chitosanase</subject><subject>cloning</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Miscellaneous</subject><subject>Mission oriented research</subject><subject>purification</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkEtv1TAQRi0Eog_6E1p5gVC7CHhix48VKlUfiEogtXvLccatUWIXO0Hi33Nvb6Quu5pZnG_m0yHkGNhnYCC_3AHXpgHV8lNjzjRjHWvEG7IPXKhGiBbebvcV2SMHtf5mDBRT8J7sgWx5K7TcJz9-LSWG6N0cc6IuDfQBE1I_5hTTA82BOuof45yrS64iDSVP9JvzcRyXSvExTphqrPTy_Bo-kHfBjRWP1nlI7q8u7y9umtuf198vzm8bLzTMjYJOa240ih76Dp3U0pmh4wYCYouehx57YYTkvTKtGPgw6A57HVyH0mh-SD7tzj6V_GfBOtspVo_j6BLmpVrFtFRg5AbsdqAvudaCwT6VOLnyzwKzW4n2WaLdGrLG2GeJVmxyJ-uDpZ9weEmt1jbAxxVw1bsxFJd8rC-cBqPVtujXHYYbGX8jFlt9xORxiAX9bIccX2nyH0kEjZI</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Akiyama, Kouichi</creator><creator>Fujita, Tadashi</creator><creator>Kuroshima, Ken-Ichi</creator><creator>Sakane, Takeshi</creator><creator>Yokota, Akira</creator><creator>Takata, Renkichi</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1</title><author>Akiyama, Kouichi ; Fujita, Tadashi ; Kuroshima, Ken-Ichi ; Sakane, Takeshi ; Yokota, Akira ; Takata, Renkichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacillus ehimensis</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>chitosanase</topic><topic>cloning</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Miscellaneous</topic><topic>Mission oriented research</topic><topic>purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akiyama, Kouichi</creatorcontrib><creatorcontrib>Fujita, Tadashi</creatorcontrib><creatorcontrib>Kuroshima, Ken-Ichi</creatorcontrib><creatorcontrib>Sakane, Takeshi</creatorcontrib><creatorcontrib>Yokota, Akira</creatorcontrib><creatorcontrib>Takata, Renkichi</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akiyama, Kouichi</au><au>Fujita, Tadashi</au><au>Kuroshima, Ken-Ichi</au><au>Sakane, Takeshi</au><au>Yokota, Akira</au><au>Takata, Renkichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>1999</date><risdate>1999</risdate><volume>87</volume><issue>3</issue><spage>383</spage><epage>385</epage><pages>383-385</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>Bacillus ehimensis EAG1 (IFO15659) produced and secreted chitosanase in the presence of exogenous chitosan. The chitosanase was purified from the culture filtrate of the bacterium to apparent homogeneity in SDS-polyacrylamide gel electrophoresis. The purified enzyme had a molecular weight of approximately 31,000. A 1.9-kbp DNA fragment containing the chitosanase gene was cloned and the complete nucleotide sequence was determined. The sequence was found to contain a single open reading frame encoding a protein of 302 amino acids. The deduced amino acid sequence showed significant homology with the chitosanase from Bacillus circulans MH-K1.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>16232486</pmid><doi>10.1016/S1389-1723(99)80050-4</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1389-1723
ispartof Journal of bioscience and bioengineering, 1999, Vol.87 (3), p.383-385
issn 1389-1723
1347-4421
language eng
recordid cdi_proquest_miscellaneous_70867196
source ScienceDirect Journals
subjects Bacillus ehimensis
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
chitosanase
cloning
Fundamental and applied biological sciences. Psychology
Miscellaneous
Mission oriented research
purification
title Purification and gene cloning of a chitosanase from Bacillus ehimensis EAG1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T04%3A37%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Purification%20and%20gene%20cloning%20of%20a%20chitosanase%20from%20Bacillus%20ehimensis%20EAG1&rft.jtitle=Journal%20of%20bioscience%20and%20bioengineering&rft.au=Akiyama,%20Kouichi&rft.date=1999&rft.volume=87&rft.issue=3&rft.spage=383&rft.epage=385&rft.pages=383-385&rft.issn=1389-1723&rft.eissn=1347-4421&rft_id=info:doi/10.1016/S1389-1723(99)80050-4&rft_dat=%3Cproquest_cross%3E70867196%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c481t-71588398e4b1b5ea686a9d5391fee2ec3fbeb49463b7924d3dd85eb8fa5e6983%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=70867196&rft_id=info:pmid/16232486&rfr_iscdi=true