Electrophoretic and immunochemical evidence showing that marsupial limb muscles express the same fast and slow myosin heavy chains as eutherians

Limb muscles of eutherian (placental) mammals express a slow and three fast isoforms of myosin heavy chain (MyHC), but little is known about marsupial MyHCs. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of limb MyHCs from seven marsupial species, spanning two orders, revealed...

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Bibliographic Details
Published in:Electrophoresis 2001-04, Vol.22 (6), p.1016-1020
Main Authors: Zhong, Wendy W. H., Lucas, Christine A., Kang, Lucia H. D., Hoh, Joseph F. Y.
Format: Article
Language:English
Subjects:
Animals
Blotting, Western - methods
Electrophoresis, Polyacrylamide Gel - methods
Extremities
Fiber type
Marsupial
Marsupialia
Muscle
Muscle, Skeletal - chemistry
Myosin heavy chain
Myosin Heavy Chains - analysis
Sodium dodecyl sulfate - polyacrylamide gel electrophoresis
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Summary:Limb muscles of eutherian (placental) mammals express a slow and three fast isoforms of myosin heavy chain (MyHC), but little is known about marsupial MyHCs. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of limb MyHCs from seven marsupial species, spanning two orders, revealed four components, each of which specifically cross‐reacted in Western blots with a monoclonal antibody (mAb) against a corresponding eutherian MyHC. For all seven species, the relative mobility of the band identified by each mAb matched that in the rat, suggesting that the four are homologous to eutherian slow, 2B, 2X and 2A MyHCs, respectively, in the order of decreasing mobility. Immunohistochemical analysis of fast marsupial limb muscles identitied four different fiber populations whose relative fiber size spectra (IIA
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683()22:6<1016::AID-ELPS1016>3.0.CO;2-K