A Novel Early Estrogen-Regulated Gene gec1 Encodes a Protein Related to GABARAP

• Published in: Biochemical and biophysical research communications 2001-06, Vol.284 (1), p.118-125
• Main Authors: Vernier-Magnin, Sandrine, Muller, Stéphanie, Sallot, Myriam, Radom, Jean, Musard, Jean-François, Adami, Pascale, Dulieu, Philippe, Rémy-Martin, Jean-Paul, Jouvenot, Michèle, Fraichard, Annick
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions; Adaptor Proteins, Signal Transducing; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; estrogen; Estrogens - pharmacology; Exons; Female; GABARAP (GABAA-receptor-associated protein), microtubule-associated protein; GABARAP protein; gec1; gec1 gene; Gene Expression Regulation - drug effects; Gene Expression Regulation - physiology; Genomic Library; genomic organization; Guinea Pigs; Humans; Introns; LD-PCR; microtubule-associated protein; Microtubule-Associated Proteins - biosynthesis; Microtubule-Associated Proteins - genetics; molecular cloning; Molecular Sequence Data; Organ Specificity; Placenta; primary response gene; RACE; RNA, Messenger - biosynthesis; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Transcription, Genetic - drug effects
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.2001.4908

We have isolated, in guinea-pig endometrial cells, an estrogen-induced 1.8 kb RNA called gec1. Screening of a guinea-pig genomic library led to identification of gec1 gene consisting of 4 exons and 3 introns. Exon 1 contains the 5′UTR and the ATG initiation codon. A guinea-pig gec1 cDNA was obtained by 5′-RACE. The 351 bp coding sequence shares 76.8% identity with that of the human GABARAP 924 bp cDNA while UTRs of the two cDNAs differ. A gec1 probe from the 3′UTR revealed a 1.9 kb mRNA in human tissues and a human GEC1 cDNA was isolated from placenta. Its coding sequence shares 93 and 79% identity with that of guinea-pig gec1 and human GABARAP, respectively. The human and guinea-pig GEC1 proteins have 100% identity. GEC1 and GABARAP proteins have 87% identity and N terminus featuring a tubulin binding motif. Thus, estrogen-regulated gec1 is a new gene which could encode a microtubule-associated protein.